Abstract
Aeromonas hydrophila is a valuable indicator of the quality of water
polluted by sewage and pathogens that pose a risk for humans and
cold-blooded animals, including fi sh. The main aim of this research was
to evaluate anthropogenic pollution of river water based on genetic
diversity of 82 A. hydrophila strains by means of RAPD, semi-random AP-PCR
(ISJ) and the rep-BOX conservative repeats test. Genetic diversity of A.
hydrophila was HT = 0.28 (SD = 0.02) for all DNA markers (RAPD, semi
random and rep-BOX). None of the analyzed electrophoretic patterns was
identical, implying that there were many sources of strain transmission.
The presence of genes for aerolysin (aerA), hemolysin (ahh1) and the
cytotoxic enzyme complex (AHCYTOGEN) was verifi ed for all tested strains,
and drug resistance patterns for tetracycline, enrofl oxacin and
erythromycin were determined. The most diverse A. hydrophila strains
isolated from river water were susceptible to enrofl oxacine (HS = 0.27),
whereas less diverse strains were susceptible to erythromycin (HS = 0.24).
The presence of the multidrug resistance marker (ISJ4-25; 1100 bp locus)
in the examined strains (resistant to three analyzed drugs) indicates that
intensive fi sh cultivation affects the microbiological quality of river
water.
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