Bacterial speck of tomato caused by Pseudomonas syringae pv. tomato appeared to be recently the most important disease on tomato in Poland. The genetic relationships among four Polish strains of race 0 P. syringae pv. tomato of different origin, isolated from tomato plants, were examined by RAPD and PCR-RFLP techniques. Amplification of bacterial DNA using 33 primers with RAPD technique showed, that similarity of strains expressed by the Nei-Li coefficient was very high (above 0.8). Next, the restriction analysis of amplified region ITS with the use of 5 endonucleases revealed, that profiles obtained from electrophoretic separation of DNA fragments were also very similar. On the basis of those analyses it was concluded that all strains tested constituted a closely related group. However, they showed various level of virulence as was demonstrated on the inoculated leaves of tomato plants growing in the greenhouse.
The present study was conducted to characterize the infectious bursal disease virus (IBDV) circulating in clinically diseased broiler chicken flocks with previous vaccination history during 2015-2016 in Egypt. IBDVs were isolated from 48 out of 63 of the investigated bursae from 10 flocks onto embryonated chicken eggs (ECEs) and verified by reverse transcriptase-poly- merase chain reaction (RT-PCR). Histopathologically, bursae lesions revealed some lymphocytes depletion as well as the presence of vesicles in the lining epithelium. The hyper variable region (HVR) of VP2 and VP1 genes of the 10 isolates (1 isolate/flock) were partially sequenced and subjected to comparative alignment and phyologenetic analysis. Phylogenetically, IBDV isolates were clustered into two distinct genetic lineages: variants of classical virulent (cv) and very viru- lent (vv) IBDV strains based on VP1 and VP2 amino acid (aa) sequences. Alignment analysis of HVR-VP2 aa sequences has demonstrated that the vvIBDV isolates have the conserved residues of the vvIBDV pathotype (A222, I242, and I256), while, the cvIBDV isolates have the same aa sequences of the classical attenuated vaccine strain (D78). Expected single point mutation occurred at position 253 (H253N). All previously characterized isolates were re-subjected to molecular analysis with VP1 protein due to its correlation with virulence and pathogenicity of IBDVs. vvIBDV isolates have the conserved tripeptide (TDN), while, the cvIBDV isolates have aa substitutions at conserved tripeptide including NEG at 145-147 amino acid. The present study has demonstrated that variants of classical virulent and very virulent IBDV circulated among vaccinated flocks in Egypt during 2015-2016.
Genotypic differentiation among 10 isolates of Phytophthora cinnamomi Rands and 24 isolates of Phytophthora citricola Sawada from 12 different plant species grown in Polish ornamental nurseries was determined. DNA was extracted from pure pathogen cultures and amplified by the PCR technique using ISSR and RAPD primers. 9 primers were used to amplify P. cinnamomi and 8 to amplify P. citricola DNA. The analyzed amplification products were between 300 and 2300 bp. The genotypical differentiation was from 17 to 35% in P. cinnamomi and from 10 to 60% in P. citricola. Isolates from host plants of the same family showed, with some exceptions, similar levels of differentiation.
Antibiotics are a group of substances potentially harmful to the environment. They can play a role in bacterial resistance transfer among pathogenic and non-pathogenic bacteria. In this experiment three representatives of medically important chemotherapeutics, confirmed to be present in high concentrations in wastewater treatment plants with HPLC analysis were used: erythromycin, sulfamethoxazole and trimethoprim. Erythromycin concentration in activated sludge was not higher than 20 ng L−1. N-acetylo-sulfamethoxazole concentration was 3349 ± 719 in winter and 2933 ± 429 ng L−1 in summer. Trimethoprim was present in wastewater at concentrations 400 ± 22 and 364 ± 60 ng L−1, respectively in winter and summer. Due to a wide variety of PCR-detectable resistance mechanisms towards these substances, the most common found in literature was chosen. For erythromycin: erm and mef genes, for sulfamethoxazole: sul1, sul2, sul3 genes, in the case of trimethoprim resistance dhfrA1 and dhfr14 were used in this study. The presence of resistance genes were analyzed in pure strains isolated from activated sludge and in the activated sludge sample itself. The research revealed that the value of minimal inhibitory concentration (MIC) did not correspond with the expected presence of more than one resistance mechanisms. Most of the isolates possessed only one of the genes responsible for a particular chemotherapeutic resistance. It was confirmed that it is possible to monitor the presence of resistance genes directly in activated sludge using PCR. Due to the limited isolates number used in the experiment these results should be regarded as preliminary.
The full-length cDNA of LeTIR1 gene was isolated from tomato with EST-based in silico cloning followed by RACE amplification. LeTIR1 contained an open reading frame (ORF) 1872 bp long, encoding 624 amino acid residues. The predicted protein LeTIR1 had one F-box motif and eleven leucine-rich repeats (LRRs), all of which are highly conserved in TIR1 proteins of other plant species. Phylogenetic analysis showed that the LeTIR1 protein shared high similarity with other known TIR1 proteins. Both sequence and phylogenetic analysis suggested that LeTIR1 is a TIR1 homologue and encodes an F-box protein in tomato. Semi-quantitative RT-PCR indicated that LeTIR1 was expressed constitutively in all organs tested, with higher expression in stem than root, leaf, flower and fruit. Its expression level was positively correlated with the auxin distribution in stem or axillary shoot, and was induced by spraying exogenous IAA.
Pseudomonas syringae pv. syringae (Pss) constitutes a diverse group of bacterial strains that cause canker of stone fruits, blight of cereals and red streak of sugarcane. The purpose of this study was to determine how diverse Iranian strains of Pss are when they come from different hosts. We compared a total of 32 Pss strains isolated from stone fruits, barley, wheat and sugarcane from different geographical regions of Iran based on their phenotypic and molecular properties. Strains showed some variation regarding carbon and nitrogen utilization. Pss strains were similar in their protein banding patterns. Additional bands were found in sugarcane strains. Most strains showed one indigenous plasmid DNA and a few had two and some none. The genes of syrB and syrD encoding syringomycin synthesis and secretion, respectively, were amplified using specific primers in polymerase chain reaction. Syringomycin, producing strains amplified two DNA fragments of 752 and 446 bp representing syrB and syrD genes, respectively. Primer specificity was shown for Pss using various genera. Based on the results of this study, it is suggested that Pss strains from different hosts and geographical regions show diversity in phenotypic and molecular characters. It is thought that phenotypic variation is due to adaptation to specific hosts and niches for survival and pathogenicity.
Perinatal calf mortality in dairy herds has been reported worldwide. The etiology of stillbirth is multifactorial, and can be caused by various species of bacteria and environmental factors. Among them some potential pathogens from the Mollicutes class such as Mycoplasma (M.) spp. and Ureaplasma (U.) diversum can be isolated from the bovine genital tract and other organs of the suspected cattle. The aim of this study was to evaluate if the bacteria belonging to the Molli- cutes class i.e. M. bovis, M. bovigenitalium, M. canadense, M. canis, M. arginini, M. bovirhinis, M. dispar, M. alkalescens and U. diversum could have an impact on perinatal calf mortality in selected Polish dairy farms. The material was: 121 stillborn calves (SB), 21 live born calves (C) and 131 cows (dams) from 30 Polish Holstein-Friesian herds. Samples were examined from all the SB calves’ and six control euthanized calves’ abomasal contents and lung samples collected during necropsy, and from the dams’ serum and placenta. In dams the serological ELISA, and in calves and placenta samples molecular PCR/denaturing gradient gel electrophoresis, methods were used. Screening of dams’ sera for antibodies to M. bovis (ELISA) showed seven dams positive for M. bovis, whereas none of the nine examined Mollicutes microorganisms were detected in the placenta and calves.
Gaeumannomyces graminis is an etiologic agent of take-all, economically important disease of cereals worldwide. A polymerase chain reaction with variety-specific primers was successfully used for detection of G. graminis var. tritici in plant tissue. Obtained results showed that this diagnostic method is a very sensitive and useful tool for detection of the pathogen even before disease symptoms arise. DNA polymorphism revealed by RAPD-PCR with three arbitrary primers was suitable for assessing genetic variation among Ggt isolates originating from wheat and rye.
Cucumber mosaic virus (CMV; family Bromoviridae, genus Cucumovirus) is the most cosmopolitan plant virus occurring worldwide. In the present study, leaf samples showing deformations, mosaics, and chlorotic spots symptoms were collected from naturally infected Basella alba, Telfairia occidentalis and Talinum fruticosum in a home yard garden in Ibadan, Nigeria. Total nucleic acid was extracted from leaves and used as template for cDNA synthesis. RT-PCR was carried out using CMV-specific primers targeting RNA-1 segment. Samples were also tested by RT-PCR using Potyvirus and Begomovirus genusspecific primers. DNA fragments with the expected sizes of ~500 bp were amplified by using CMV-specific primers; however, the expected amplicons were not produced using specific primers used for the detection of potyviruses and begomoviruses. The nucleotide and deduced amino acid sequences obtained for the isolates studied contained 503–511 nt and 144 aa, respectively. The isolates shared 81.9–85.3% nucleotide and 74.3–77.8% amino acid sequence identities with each other. The results of BLASTN analyses showed the highest identities of the isolates (80–93%) with CMV strains from Japan, USA and South Korea. Alignment of deduced partial protein revealed multiple amino acid substitutions within the three isolates and high identities with CMV subgroup I. Phylogenetic analyses putatively categorized the isolates in close association with subgroup IB isolates. The three isolates clustered together into a separate subclade, indicating possible new CMV strains. The results provide the first molecular evidence for CMV infections of T. fruticosum and B. alba in Nigeria and seem to show the possible presence of new strain(s). These findings also add three new hosts to the list of natural host range of the virus in Nigeria.
Canine babesiosis is a tickborne, protozoal, haemoparasitic disease. Babesia organisms are frequently classified as either large (B. canis) or small (B. gibsoni). The aim of this study was an attempt to detect B. gibsoni DNA in blood samples taken from dogs suspected of suffering from tick-borne diseases. 216 samples were tested using PCR, of which, in 99 of them B. canis DNA was detected, whereas in 3 of them B. gibsoni was detected. Positive PCR results for B. gibsoni were confirmed using a Qube MDx real-time analyzer. The results indicate that infections with this B. gibsoni should be taken into account and included in the differential diagnosis of vector-borne diseases in dogs in Poland, and that the accurate identification of the species of parasite causing the infection is crucial for developing the correct treatment regimen and prognosis.
Cryptosporidium spp. is a protozoan parasite of many vertebrates worldwide including avian hosts, causing gastroenteritis and diarrhea. Studies have been conducted on Cryptosporidium spp. in some avians, however, there is no information on Cryptosporidium spp. in pigeons from Anhui Province, China. To investigate the prevalence and assess the transmission burden of Cryptosporidium species in domestic pigeons, a total of 376 fecal samples were collected. The acid-fast staining and nested PCR amplification methods reveal a Cryptosporidium prevalence rate of 5.05% (19/376) and 1.86% (7/376), respectively. Furthermore, molecular characterization was identified as Cryptosporidium meleagridis. As this study is the first report on Cryptosporidium spp. in domestic pigeons in Anhui Province, we expect it to provide baseline information for further studies.
Bouzid M, Hunter PR, Chalmers RM, Tyler KM (2013) Cryptosporidium pathogenicity and virulence. Clin Microbiol Rev 26: 115-134.
Briceño C, Marcone D, Larraechea M, Hidalgo H, Fredes F, Ramírez-Toloza G, Cabrera G (2023) Zoonotic Cryptosporidium meleagridis in urban invasive monk parakeets. Zoonoses Public Health 70: 705-710.
Cama VA, Bern C, Roberts J, Cabrera L, Sterling CR, Ortega Y, Gilman RH, Xiao L (2008) Cryptosporidium species and subtypes and clinical manifestations in children, Peru. Emerg Infect Dis 14: 1567-74.
Cama VA, Ross JM, Crawford S, Kawai V, Chavez-Valdez R, Vargas D, Vivar A, Ticona E, Navincopa M, Williamson J, Ortega Y, Gilman RH, Bern C, Xiao L (2007) Differences in clinical manifestations among Cryptosporidium species and subtypes in HIV-infected persons. J Infect Dis 196: 684-691.
Couso-Pérez S, Ares-Mazás E, Gómez-Couso H (2022) A review of the current status of Cryptosporidium in fish. Parasitology 149: 1-13.
Dąbrowska J, Sroka J, Cencek T (2023) Investigating Cryptosporidium spp. Using Genomic, Proteomic and Transcriptomic Techniques: Current Progress and Future Directions. Int J Mol Sci 24: 12867.
Dong HJ, Cheng R, Li XM, Li J, Chen YC, Ban CP, Zhang XQ, Liu F, Zhang LX (2021) Molecular Identification of Cryptosporidium spp., Enterocytozoon bieneusi, and Giardia duodenalis in Captive Pet Birds in Henan Province, Central China. J Eukaryot Microbiol 68: e12839.
Elliot A, Morgan UM, Thompson RC (1999) Improved staining method for detecting Cryptosporidium oocysts in stools using malachite green. J Gen Appl Microbiol 45: 139-142.
Feng X, Tuo H, Li T, Yu F, Hu D, Yang X, Ge Y, Qi M, Liu X (2022) Longitudinal surveillance of Cryptosporidium spp. in broiler chickens in Xinjiang, northwest China: genetic diversity of Cryptosporidium meleagridis subtypes. Parasitol Res 121: 3589-3595.
Fujino T, Matsuo T, Okada M, Matsui T (2006) Detection of a small number of Cryptosporidium parvum oocysts by sugar flotation and sugar centrifugation methods. J Vet Med Sci 68: 1191-1193.
Garcia-R JC, Pita AB, Velathanthiri N, Pas A, Hayman DT (2023) Mammal-related Cryptosporidium infections in endemic reptiles of New Zealand. Parasitol Res 122: 1239-1244.
Gattan HS, Alshammari A, Marzok M, Salem M, Al-Jabr OA, Selim A (2023) Prevalence of Cryptosporidium infection and associated risk factors in calves in Egypt. Sci Rep 13: 17755.
Golomazou E, Malandrakis EE, Panagiotaki P, Karanis P (2021) Cryptosporidium in fish: Implications for aquaculture and beyond. Water Res 201: 117357.
Hallinger MJ, Taubert A, Hermosilla C (2020) Endoparasites infecting exotic captive amphibian pet and zoo animals (Anura, Caudata) in Germany. Parasitol Res 119: 3659-3673.
Holubová N, Sak B, Horčičková M, Hlásková L, Květoňová D, Menchaca S, McEvoy J, Kváč M (2016) Cryptosporidium avium n. sp. (Apicomplexa: Cryptosporidiidae) in birds. Parasitol Res 115: 2243-2251.
Holubová N, Zikmundová V, Kicia M, Zajączkowska Ż, Rajský M, Konečný R, Rost M, Mravcová K, Sak B, Kváč M (2024) Genetic diversity of Cryptosporidium spp., Encephalitozoon spp. and Enterocytozoon bieneusi in feral and captive pigeons in Central Europe. Parasitol Res 123: 158.
Kabir MHB, Han Y, Lee SH, Nugraha AB, Recuenco F, Murakoshi F, Xuan X, Kato K (2020) Prevalence and molecular characterization of Cryptosporidium species in poultry in Bangladesh. One Health 9: 100122.
Kirkpatrick CE (1985) Cryptosporidium infection as a cause of calf diarrhea. Vet Clin North Am Food Anim Pract 1: 515-528.
Koompapong K, Mori H, Thammasonthijarern N, Prasertbun R, Pintong AR, Popruk S, Rojekittikhun W, Chaisiri K, Sukthana Y, Mahittikorn A (2014) Molecular identification of Cryptosporidium spp. in seagulls, pigeons, dogs, and cats in Thailand. Parasite 21: 52.
Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol 33: 1870-1874.
Li J, Lin XH, Zhang L, Qi N, Liao S, Lv M, Wu C, Sun M (2015) Molecular characterization of Cryptosporidium spp. in domestic pigeons (Columba livia domestica) in Guangdong Province, Southern China. Parasitol Res 114: 2237-2241.
Liao C, Wang T, Koehler AV, Hu M, Gasser RB (2021) Cryptosporidium of birds in pet markets in Wuhan city, Hubei, China. Curr Res Parasitol Vector Borne Dis 1: 100025.
Lin XH, Xin LY, Qi M, Hou MY, Liao SQ, Qi NS, Li J, Lv MN, Cai HM, Hu JJ, Zhang J, Ji XB, Sun MF (2022) Dominance of the zoonotic pathogen Cryptosporidium meleagridis in broiler chickens in Guangdong, China, reveals evidence of cross-transmission. Parasit Vectors 15: 188.
Meamar AR, Guyot K, Certad G, Dei-Cas E, Mohraz M, Mohebali M, Mohammad K, Mehbod AA, Rezaie S, Rezaian M (2007) Molecular characterization of Cryptosporidium isolates from humans and animals in Iran. Appl Environ Microbiol 73: 1033-1035.
Messa A Jr, Köster PC, Garrine M, Nhampossa T, Massora S, Cossa A, Bassat Q, Kotloff K, Levine MM, Alonso PL, Carmena D, Mandomando I (2021) Molecular Characterisation of Cryptosporidium spp. in Mozambican Children Younger than 5 Years Enrolled in a Matched Case-Control Study on the Aetiology of Diarrhoeal Disease. Pathogens 10: 452.
Mohamed MA, Hammam HM, El-Taweel HA, Abd El-Latif NF (2022) Cryptosporidium species in HIV patients in Alexandria, Egypt: distribution and associated clinical findings. Trop Biomed 39: 108-116.
Moratal S, Dea-Ayuela MA, Cardells J, Marco-Hirs NM, Puigcercós S, Lizana V, López-Ramon J (2020) Potential Risk of Three Zoonotic Protozoa (Cryptosporidium spp., Giardia duodenalis, and Toxoplasma gondii) Transmission from Fish Consumption. Foods 9:1913.
Mulunda NR, Hayashida K, Yamagishi J, Sianongo S, Munsaka G, Sugimoto C, Mutengo MM (2020) Molecular characterization of Cryptosporidium spp. from patients with diarrhoea in Lusaka, Zambia. Parasite 27: 53.
Nakamura AA, Meireles MV (2015) Cryptosporidium infections in birds – a review. Rev Bras Parasitol Vet 24: 253-67.
O’Donoghue PJ, Tham VL, de Saram WG, Paull KL, McDermott S (1987) Cryptosporidium infections in birds and mammals and attempted cross-transmission studies. Vet Parasitol 26:1-11.
Pedraza-Díaz S, Ortega-Mora LM, Carrión BA, Navarro V, Gómez-Bautista M (2009) Molecular characterisation of Cryptosporidium isolates from pet reptiles. Vet Parasitol 160: 204-210.
Radfar MH, Asl EN, Seghinsara HR (2012) Biodiversity and prevalence of parasites of domestic pigeons (Columba livia domestica) in a selected semiarid zone of South Khorasan, Iran. Trop Anim Health Prod 44:225-229.
Robertson LJ, Johansen ØH, Kifleyohannes T (2020) Cryptosporidium Infections in Africa-How Important Is Zoonotic Transmission? A Review of the Evidence. Front Vet Sci 7:575881.
Ryan U (2010) Cryptosporidium in birds, fish and amphibians. Exp Parasitol 124: 113- 120.
Ryan U, Power M (2012) Cryptosporidium species in Australian wildlife and domestic animals. Parasitology 139: 1673-1688.
Sannella AR, Suputtamongkol Y, Wongsawat E, Cacciò SM (2019) A retrospective molecular study of Cryptosporidium species and genotypes in HIV-infected patients from Thailand. Parasit Vectors 12: 91.
Seixas M, Taroda A, Cardim ST, Sasse JP, Martins TA, Martins FDC, Minutti AF, Vidotto O, Barros LD, Garcia JL (2019) First study of Cryptosporidium spp. occurrence in eared doves (Zenaida auriculata). Rev Bras Parasitol Vet 28: 489-492.
Silverlås C, Mattsson JG, Insulander M, Lebbad M (2012) Zoonotic transmission of Cryptosporidium meleagridis on an organic Swedish farm. Int J Parasitol 42:963- 967.
Slavin D (1955) Cryptosporidium meleagridis (sp. nov.). J Comp Pathol 65: 262-266.
Tamura K, Stecher G, Kumar S (2021) MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Mol Biol Evol 38: 3022-3027.
Tzipori S (1988) Cryptosporidiosis in perspective. Adv Parasitol 27: 63-129.
Upton SJ, McAllister CT, Freed PS, Barnard SM (1989) Cryptosporidium spp. in wild and captive reptiles. J Wildl Dis 25: 20-30.
Wang W, Wei Y, Cao S, Wu W, Zhao W, Guo Y, Xiao L, Feng Y, Li N (2022) Divergent Cryptosporidium species and host-adapted Cryptosporidium canis subtypes in farmed minks, raccoon dogs and foxes in Shandong, China. Front Cell Infect Microbiol 12: 980917.
Wang Y, Zhang K, Chen Y, Li X, Zhang L (2021) Cryptosporidium and cryptosporidiosis in wild birds: A One Health perspective. Parasitol Res 120: 3035-3044.
Xiao L, Fayer R, Ryan U, Upton SJ (2004) Cryptosporidium taxonomy: recent advances and implications for public health. Clin Microbiol Rev 17: 72-97.
Xiao L, Feng Y (2008) Zoonotic cryptosporidiosis. FEMS Immunol Med Microbiol 52:309-323.
Xiao L, Morgan UM, Limor J, Escalante A, Arrowood M, Shulaw W, Thompson RC, Fayer R, Lal AA (1999) Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. Appl Environ Microbiol 65: 3386-3391.
Wang Y, Yang W, Cama V, Wang L, Cabrera L, Ortega Y, Bern C, Feng Y, Gilman R, Xiao L (2014) Population genetics of Cryptosporidium meleagridis in humans and birds: evidence for cross-species transmission. Int J Parasitol 44: 515-21.
Zaglool DA, Mohamed A, Khodari YA, Farooq MU (2013) Crypto-Giardia antigen rapid test versus conventional modified Ziehl-Neelsen acid fast staining method for diagnosis of cryptosporidiosis. Asian Pac J Trop Med 6: 212-215.
Zhang Y, Lu Z, Liu Z, Zhou Y, Xiao G, Opeyemi AO, Jin S, Li Y, Liu T, Wu Q, Sun X, Xu Q, Zhang Q, Yang C (2024) Prevalence and molecular characterization of Trichomonas gallinae from pigeons in Anhui, China. Comp Immunol Microbiol Infect Dis 107: 102157.
AbuOun M, Stubberfield EJ, Duggett NA, Kirchner M, Dormer L, NunezGarcia J, Randall LP, Lemma F, Crook DW, Teale C, Smith RP, Anjum MF (2017) mcr-1 and mcr-2 variant genes identified in Moraxella species isolated from pigs in Great Britain from 2014 to 2015. J Antimicrob Chemother 72: 2745-2749.
Adiguzel MC, Baran A, Wu Z, Cengiz S, Dai L, Oz C, Ozmenli E, Goulart DB, Sahin O (2021) Prevalence of colistin resistance in Escherichia coli in eastern Turkey and genomic characterization of an mcr-1 positive strain from retail chicken meat. Microb Drug Resist 27: 424-432.
Alba P, Leekitcharoenphon P, Franco A, Feltrin F, Ianzano A, Caprioli A, Stravino F, Hendriksen RS, Bortolaia V, Battisti A (2018) Molecular epidemiology of mcr-encoded colistin resistance in Enterobacteriaceae from food-producing animals in Italy revealed through the EU harmonized antimicrobial resistance monitoring. Front Microbiol 9: 1217.
Al-Bayssari C, Dabboussi F, Hamze M, Rolain JM (2015) Detection of expanded-spectrum β-lactamases in Gram-negative bacteria in the 21st century. Expert Rev Anti Infect Ther 13: 1139-1158.
Ayaz ND, Cufaoglu G, Yonsul Y, Goncuoglu M, Erol I (2019) Plasmid-mediated colistin resistance in Escherichia coli O157:H7 cattle and sheep isolates and whole-genome sequence of a colistin-resistant sorbitol fermentative Escherichia coli O157:H7. Microb Drug Resist 25: 1497-1506.
Babacan O (2023) First detection of carbapenem resistance in Enterobacteriaceae isolates isolated from dairy cows’ mastitis infection in Türkiye. Ankara Univ Vet Fak Derg 70: 65-74.
Bhoomika SS, Patyal A, Gade NE (2016) Occurrence and characteristics of extended-spectrum β-lactamases producing Escherichia coli in foods of animal origin and human clinical samples in Chhattisgarh, India. Vet World 9: 996-1000.
Borowiak M, Baumann B, Fischer J, Thomas K, Deneke C, Hammerl JA, Szabo I, Malorny B (2020) Development of a novel mcr-6 to mcr-9 multiplex PCR and Assessment of mcr-1 to mcr-9 occurrence in colistin-resistant Salmonella enterica isolates. From environment, feed, animals and food (2011-2018) in Germany. Front Microbiol 11: 80.
Borowiak M, Fischer J, Hammerl JA, Hendriksen RS, Szabo I, Malorny B (2017) Identification of a novel transposon-associated phosphoethanolamine transferase gene, mcr-5, conferring colistin resistance in d-tartrate fermenting Salmonella enterica subsp. enterica serovar Paratyphi B. J Antimicrob Chemother 72: 3317-3324.
Braun SD, Ahmed MFE, El-Adawy H, Hotzel H, Engelmann I, Weiß D, Monecke S, Ehricht R (2016) Surveillance of extended-spectrum beta-lactamase-producing Escherichia coli in dairy cattle farms in the Nile Delta, Egypt. Front Microbiol 7: 1020.
Carattoli A, Villa L, Feudi C, Curcio L, Orsini S, Luppi A, Pezzotti G, Magistrali CF (2017) Novel plasmid-mediated colistin resistance mcr-4 gene in Salmonella and Escherichia coli, Italy 2013, Spain and Belgium, 2015 to 2016. Euro Surveill 22: 30589.
Carretto E, Brovarone F, Nardini P, Russello G, Barbarini D, Pongolini S, Gagliotti C, Carattoli A, Sarti M (2018) Detection of mcr-4 positive Salmonella enterica serovar Typhimurium in clinical isolates of human origin, Italy, October to November 2016. Eur Surveill 23: 17-00821.
Carroll LM, Gaballa A, Guldimann C, Sullivan G, Henderson LO, Wiedmann M (2019) Identification of novel mobilized colistin resistance gene mcr-9 in a multidrugresistant, colistin-susceptible Salmonella enterica serotype Typhimurium isolate. mBio 10: e00853-19.
Carvalho IT, Santos L (2016) Antibiotics in the aquatic environments: a review of the European scenario. Environ Int 94: 736-757.
Cornaglia G, Akova M, Amicosante G, Cantón R, Cauda R, Docquier JD, Edelstein M, Frère JM, Fuzi M, Galleni M, Giamarellou H, Gniadkowski M, Koncan R, Libisch B, Luzzaro F, Miriagou V, Navarro F, Nordmann P, Pagani L, Peixe L, Poirel L, Souli M, Tacconelli E, Vatopoulos A, Rossolini GM; ESCMID Study Group for Antimicrobial Resistance Surveillance (ESGARS) (2007) Metallo-beta-lactamases as emerging resistance determinants in Gram-negative pathogens: open issues. Int J Antimicrob Agents 29: 380-388.
Dubois D, Grare M, Prere MF, Segonds C, Marty N, Oswald E (2012) Performances of the Vitek MS matrix-assisted laser desorption ionization – time of flight mass spectrometry system for rapid identification of bacteria in routine clinical microbiology. J Clin Microbiol 50: 2568-2576.
Duman ve Tekerekoğlu A (2020) Colistin MICs and resistance genes of Acinetobacter Baumannii ısolated in ıntensive care units. Turk J Intensive Care DOI: 10.4274/tybd.galenos. 2020.47965.
Ellington MJ, Kistler J, Livermore DM, Woodford N (2007) Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases. J Antimicrob Chemother 59: 321-322.
EUCAST, European Committee on Antimicrobial Susceptibility Testing (2021) Breakpoint tables for ınterpretation of MICs and zone diameters, EUCAST, Version 11.1. https://www.eucast.org/mic_and_zone_distributions_and_ecoffs
Gurung S, Kafle S, Dhungel B, Adhikari N, Thapa Shrestha U, Adhikari B, Banjara MR, Rijal KR, Ghimire P (2020) Detection of OXA-48 gene in carbapenem-resistant Escherichia coli and Klebsiella pneumoniae from urine smples. Infect Drug Resist 13: 2311-2321.
Güzel M, Avşaroğlu MD, Soyer Y (2020) Determination of colistin resistance in Escherichia coli isolates from foods in Turkey, 2011-2015. Food Health 6: 160-169.
Haenni M, Beyrouthy R, Lupo A, Chatre P, Madec JY, Bonnet R (2018) Epidemic spread of Escherichia coli ST744 isolates carrying mcr-3 and blaCTX- M-55 in cattle in France. J Antimicrob Chemother 73: 533-536.
Hasman H, Mevius D, Veldman K, Olesen I, Aarestrup FM (2005) beta-Lactamases among extended-spectrum beta-lactamase (ESBL)-resistant Salmonella from poultry, poultry products and human patients in The Netherlands. J Antimicrob Chemother 56: 115-121.
Hassan J, Eddine RZ, Mann D, Li S, Deng X, Saoud IP, Kassem II (2020) The mobile colistin resistance gene, mcr-1.1, ıs carried on IncX4 plasmids in multi-drug resistant E. coli ısolated from rainbow trout aquaculture. Microorganisms 23: 1636.
Hernandez M, Iglesias MR, Rodriguez-Lázaro D, Gallardo A, Quijada N, Miguela-Villoldo P, Campos MJ, Piriz S, Lopez-Orozco G, de Frutos C, Saez JL, Ugarte-Ruiz M, Dominguez L, Queseda A (2017) Co-occurrence of colistin-resistance genes mcr-1 and mcr-3 among multidrug-resistant Escherichia coli isolated from cattle, Spain, September 2015. Euro Surveill 22: 30586.
Huang X, Yu L, Chen X, Zhi C, Yao X, Liu Y, Wu S, Guo Z, Yi L, Zeng Z, Liu JH (2017) High prevalence of colistin resistance and mcr-1 gene in Escherichia coli isolated from food animals in China. Front Microbiol 8: 562.
Kawanishi M, Abo H, Ozawa M, Uchiyama M, Shirakawa T, Suzuki S, Shima A, Yamashita A, Sekizuka T, Kato K, Kuroda M, Koike R, Kijimaet M (2017) Prevalence of colistin resistance gene mcr-1 and absence of mcr-2 in Escherichia coli isolated from healthy food-producing animals in Japan. Antimicrob Agents Chemothe 61: e02057-16.
Khalifa HO, Ahmed AM, Oreiby AF, Eid AM, Shimamoto T, Shimamoto T (2016) Characterisation of the plasmidmediated colistin resistance gene mcr-1 in Escherichia coli isolated from animals in Egypt. Int J Antimicrob Agents 47: 413-414.
Kilianski, A, Haas JL, Corriveau EJ, Liem AT, Willis KL, Kadavy DR, Rosenzweig CN, Minot SS (2015) Bacterial and viral identification and differentiation by amplicon sequencing on the MinION nanopore sequencer. Gigascience 4: 12.
Kuenzli E (2016) Antibiotic resistance and international travel: causes and consequences. Travel Med Infect Dis 14: 595-598.
Kurekci C, Aydin M, Nalbantoglu OU, Gundogdu A (2018) First report of Escherichia coli carrying the mobile colistin resistance gene mcr-1 in Turkey. J Glob Antimicrob Resist. 15: 169-170.
Laxminarayan R, Matsoso P, Pant S, Brower C, Røttingen JA, Klugman K, Davies S (2016) Access to effective antimicrobials: a worldwide challenge. Lancet 387: 168-175.
Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R, Spencer J, Doi Y, Tian G, Dong B, Huang X, Yu LF, Gu D, Ren H, Chen X, Lv L, He D, Zhou H, Liang Z, Liu JH, Shen J (2016) Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis 16: 161-168.
Luo Q, Wang Y, Xiao Y (2020) Prevalence and transmission of mobilized colistin resistance (mcr) gene in bacteria common to animals and humans. Biosaf Health 2: 71-78.
McEachran AD, Blackwell BR, Hanson JD, Wooten KJ, Mayer GD, Cox SB, Smith PN (2015) Antibiotics, bacteria, and antibiotic resistance genes: aerial transport from cattle feed yards via particulate matter. Environ Health Perspect 123: 337-343.
Nakano A, Nakano R, Nishisouzu R, Suzuki Y, Horiuchi S, Kikuchi-Ueda T, Ubagai T, Ono Y, Yano H (2021) Prevalence and Relatedness of mcr-1-Mediated colistin-resistant Escherichia coli ısolated from livestock and farmers in Japan. Front. Microbiol 12: 664931.
Nicolaou KC, Rigol S (2018) A brief history of antibiotics and select advances in their synthesis. J Antibiot (Tokyo) 71: 153-184.
Otlu B, Yakupoğulları Y, Gürsoy NC, Duman Y, Bayındır Y, Tekerekoğlu MS, Ersoy Y (2018) Co-production of OXA-48 and NDM-1 Carbapenemases in Providencia rettgeri: the first report. Mikrobiyol Bul 52: 300-307.
Özkaya E, Buruk CK, Tosun İ, Toraman B, Kaklıkkaya N, Aydın F (2020) Investigation of plasmid mediated mcr colistin resistance gene in clinical Enterobacterales isolates. Mikrobiyol Bul 54:191-202.
Pai H, Lyu S, Lee JH, Kim J, Kwon Y, Kim JW, Choe KW (1999) Survey of extended-spectrum β-lactamases in clinical isolates of Escherichia coli and Klebsiella pneumoniae: prevalence of TEM-52 in Korea. J Clin Microbiol 37: 1758-1763.
Patel G, Bonomo RA (2013) Stormy waters ahead: global emergence of carbapenemases. Front Microbiol 4: 48.
Poirel L, Naas T, Nordmann P (2010) Diversity, epidemiology, and genetics of class D beta-lactamases. Antimicrob Agents Chemother 54: 24-38.
Poirel L, Walsh TR, Cuvillier V, Nordmann P (2011) Multiplex PCR for detection of acquired carbapenemase genes. Diagn Microbiol Infect Dis 70: 119-123.
Queenan AM, Bush K (2007) Carbapenemases: the versatile beta-lactamases. Clin Microbiol Rev 20: 440-458.
Rebelo AR, Bortolaia V, Kjeldgaard JS, Pedersen SK, Leekitcharoenphon P, Hansen IM, Guerra B, Malorny B, Borowiak M, Hammerl JA, Battisti A, Franco A, Alba P, Perrin-Guyomard A, Granier SA, De Frutos Escobar C, Malhotra-Kumar S, Villa L, Carattoli A, Hendriksen RS (2018) Multiplex PCR for detection of plasmid-mediated colistin resistance determinants, mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5 for surveillance purposes. Euro Surveill 23: 17-00672.
Sarı AN, Süzük S, Karatuna O, Öğünç D, Karakoç AE, Çizmeci Z, Alışkan HE, Cömert F, Bakıcı MZ, Akpolat N, Çilli FF, Zer Y, Karataş A, Akgün Karapınar B, Bayramoğlu G, Özdamar M, Kalem F, Delialioğlu N, Aktaş E, Yılmaz N, Gürcan S, Gülay Z (2017) Results of a multicenter study ınvestigating plasmid mediated colistin resistance genes (mcr-1 and mcr-2) in clinical Enterobacteriaceae ısolates from Turkey. Mikrobiyol Bul 51: 299-303.
Skov RL, Monnet DL (2016) Plasmid-mediated colistin resistance (mcr-1 gene): three months later, the story unfolds. Eurosurveill 21: 30155
Sturenburg E, Kühn A, Mack D, Laufs R (2004) A novel extended-spectrum β-lactamase CTX-M-23 with a P167T substitution in the active-site omega loop associated with ceftazidime resistance. J Antimicrobial Chemother 54: 406-409.
Sun J, Zeng X, Li XP, Liao XP, Liu YH, Lin J (2017) Plasmid-mediated colistin resistance in animals: current status and future directions. Anim Health Res Rev 18: 136-152.
TEPAV (2019) Antimicrobial resistance in Turkey: Economic evaluation and recommendations. TEPAV: 1-30. https://www.tepav.org.tr/upload/files/1504774735-1.Turkiye’de Antimikrobiyal Direnç Ekonomik Degerlendirme ve Oneriler.pdf.
Wang C, Feng Y, Liu L, Wei L, Kang M, Zong Z (2020) Identification of novel mobile colistin resistance gene mcr-10. Emerg Microb Infect 9: 508-516.
Wang R, van Dorp L, Shaw LP, Bradley P, Wang Q, Wang X, Jin L, Zhang Q, Liu Y, Rieux A (2018) The global distribution and spread of the mobilized colistin resistance gene mcr-1, Nat Communi 9: 1179.
Wang X, Wang Y, Zhou Y, Wang Z, Wang Y, Zhang S, Shen Z (2019) Emergence of colistin resistance gene mcr-8 and its variant in Raoultella ornithinolytica. Fron Microbiol 10: 228.
Westblade LF, Jennemann R, Branda JA, Bythrow M, Ferraro MJ, Garner OB, Ginocchio CC, Lewinski MA, Manji R, Mochon AB, Procop GW, Richter SS, Rychert JA, Sercia L, Burnham CA (2013) Multicenter study evaluating the Vitek MS system for identification of medically important yeasts. J Clin Microbiol 51: 2267-2272.
WHO. World Health Organization (2021) Antibiotic resistance, https://www.who.int/news-room/fact-sheets/detail/antibio
ticresistance.
Xavier BB, Lammens C, Ruhal R, Kumar-Singh S, Butaye P, Goossens H, Malhotra-Kumar S (2016) Identification of a novel plasmid-mediated colistin-resistance gene, mcr-2, in Escherichia coli, Belgium, June 2016. Eurossurveill 21: 30280.
Yang YQ, Li YX, Lei CW, Zhang AY, Wang HN (2018) Novel plasmid-mediated colistin resistance gene mcr-7.1 in Klebsiella pneumonia. J Antimicrob Chemother 73: 1791-1795.
Yin W, Li H, Shen Y, Liu Z, Wang S, Shen Z, Zhang R, Walsh TR, Shen J, Wang Y (2017) Novel plasmid-mediated colistin resistance gene mcr-3 in Escherichia coli. mBio 8: e00543-17.
Yong JW, Ge L, Ng YF, Tan SN (2009) The chemical composition and biological properties of coconut (Cocos nucifera L.) water. Molecules 14: 5144-5164.
Zając M, Sztromwasser P, Bortolaia V, Leekitcharoenphon P, Cavaco LM, Ziȩtek-Barszcz A, Hendriksen RS, Wasyl D (2019) Occurrence and characterization of mcr-1-positive Escherichia coli isolated from food-producing animals in Poland, 2011-2016. Front Microbiol 10: 1753.
Zhang X, Zhang B, Guo Y, Wang J, Zhao P, Liu J, He K (2019) Colistin resistance prevalence in Escherichia coli from domestic animals in intensive breeding farms of Jiangsu Province. Int J Food Microbiol 291: 87-90.
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