@ARTICLE{Wang_Yi-Chieh_Thidiazuron_2018,
 author={Wang, Yi-Chieh and Lin, Meng-Ze and Huang, Bin and Chung, Hsiao-Hang and Chen, Jen-Tsung},
 volume={vol. 60},
 number={No 2},
 journal={Acta Biologica Cracoviensia s. Botanica},
 pages={35–44},
 howpublished={online},
 year={2018},
 publisher={Biological Commission of the Polish Academy of Sciences – Cracow Branch},
 abstract={An efficient system of micropropagation via somatic embryogenesis from root-derived callus was established in Arabica coffee (Coffea arabica L.). Twenty-six callus lines were induced on MS (Murashige and Skoog, 1962) medium supplemented with combinations of NAA (0, 0.1, 0.5, 1 and 2 mg/L) plus BA (0, 1 and 2 mg/L), or 2,4-D (0, 0.1, 0.5, 1 and 2 mg/L) plus TDZ (0, 1 and 2 mg/L). Subsequently, two types of somatic embryos were obtained from callus cultures and named S-type and I-type embryos. The S-type embryos were obtained from an 18-monthold callus line which was induced and maintained at 2 mg/L TDZ and 0.1 mg/L 2,4-D near the end of each period of the subculture. These embryos have a developmental barrier, which did not pass through the torpedo stage and could be overcome by a supplement of 2 or 5 mg/L BA. The I-type embryos were induced from 3-month-old callus when transferred onto induction media, i.e., MS supplemented with TDZ (2 and 5 mg/L) plus 2,4-D (0 and 0.1 mg/L). The significantly highest response, i.e., 13.3 embryos per callus clump was obtained at 2 mg/L TDZ. In this study, the results reveal that TDZ has a crucial effect on embryogenic callus induction, proliferation and subsequent somatic embryogenesis.},
 type={Artykuły / Articles},
 title={Thidiazuron Enhanced Somatic Embryogenes is from Callus Lines of Arabica Coffee and Subsequent Plant Regeneration},
 URL={http://ochroma.man.poznan.pl/Content/102670/PDF/ABC%20Bot.%202-18%204-Y.Wang%20i%20in.pdf},
 doi={10.24425/118053},
 keywords={callus, developmental barrier, embryogenic capacity, somatic embryo},
}