@ARTICLE{Turkiewicz_Marianna_Invertase_2005,
 author={Turkiewicz, Marianna and Pazgier, Marzena and Donachie, Stuart P. and Kalinowska, Halina},
 volume={vol. 26},
 number={No 2},
 journal={Polish Polar Research},
 pages={125-136},
 howpublished={online},
 year={2005},
 publisher={Polish Academy of Sciences},
 publisher={Committee on Polar Research},
 abstract={The marine psychrophilic and endemic Antarctic yeast Leucosporidium antarcticum strain 171 synthesizes intracellular b-fructofuranosidase, and intra- and extracellular a-glucosidases. Each enzyme is maximally produced at 5°C , while the strain’s optimum growth temperature is 15°C . Invertase biosynthesis appeared regulated by catabolic repression, and induced by sucrose; the enzyme was extremely unstable ex vivo, and only EDTA, Mn2+, and BSA stabilized it for up to 12 h after yeast cell lysis. Thermal stability of the invertase was also low (30 min at temperatures up to 12°C). The optimum temperature for invertase activity was 30°C , and optimum pH was 4.55 to 4.75. The extracellular a-glucosidase was maximally active at 35°C and pH 6.70–7.50, and stable for 30 min up to 20°C.},
 type={Article},
 title={Invertase and a-glucosidase production by the endemic Antarctic marine yeast Leucosporidium antarcticum},
 URL={http://ochroma.man.poznan.pl/Content/110633/PDF-MASTER/ppr26-125.pdf},
 keywords={Antarctica, invertase, a-glucosidase, psychrophile yeast, Leucosporidium antarcticum},
}