@ARTICLE{Xin-qin_J._Expression_2020,
 author={Xin-qin, J. and Biao, W. and Zhi-qiang, D. and Yong, R. and Yan, H. and Meng-qiu, H. and Shan-shan, D. and Yu-jie, T.},
 volume={vol. 23},
 number={No 3},
 journal={Polish Journal of Veterinary Sciences},
 pages={423-430},
 howpublished={online},
 year={2020},
 publisher={Polish Academy of Sciences Committee of Veterinary Sciences},
 publisher={University of Warmia and Mazury in Olsztyn},
 abstract={An eukaryotic expression system of Congjiang pigs IFN-λ1 was constructed to obtain its expression in CHO-K1 cells and the inhibition effect of Congjiang pig IFN-λ1 on PRRSV proliferation was verified. The eukaryotic expression plasmid pEGFP-PoIFN-λ1 was constructed from the pig IFN-λ1 gene fragment and transfected into CHO-K1 cells. Expression was detected by fluorescence microscopy and Western blotting. The influence on the proliferation of PRRSV was assessed. The results of the study showed that the recombinant plasmid pEGFP-PoIFN-λ1 was constructed correctly. After transfection, green fluorescent signal was detected in CHO-K1 cells by fluorescence microscopy. Western blot analysis revealed that in cells at different time periods after transfection, porcine IFN-λ1 was expressed, with the highest expression observed 36 h after transfection. The antiviral activity of the supernatant after 36 h of transfection was determined by the micro cytopathic inhibition method, and the biological activity was 2.1×103 U/mL. Quantitative PCR was used to detect the proliferation of PRRSV, and the results showed that Congjiang pigs IFN-λ1 significantly inhibited the mRNA expression of PRRSV and viral proliferation in a dose- and time-dependent manner. This study established a Congjiang pig IFN-λ1 eukaryotic expression system, and the quantitative PCR method showed that it has a significant inhibitory effect on the proliferation of PRRSV, which lays a foundation for the future production of antiviral drugs and clinical application.},
 type={Article},
 title={Expression of IFN-λ1 from Congjiang pigs and its effect on anti-PRRSV proliferation},
 URL={http://ochroma.man.poznan.pl/Content/117719/PDF/12.pdf},
 doi={10.24425/pjvs.2020.134687},
 keywords={porcine ifn-λ1, porcine type III interferon, eukaryotic expression, anti-proliferation},
}