@ARTICLE{Antecka_Anna_Separation_2024,
 author={Antecka, Anna and Szeląg, Rafał and Ledakowicz, Stanisław},
 volume={vol. 45},
 number={No 2},
 journal={Chemical and Process Engineering: New Frontiers},
 pages={e62},
 howpublished={online},
 year={2024},
 publisher={Polish Academy of Sciences Committee of Chemical and Process Engineering},
 abstract={The use of foam fractionation followed by aqueous two-phase extraction has emerged as a potential alternative to traditional liquid chromatography, hitherto irreplaceable in the purification of phycobiliproteins. The crude extracts of C-phycocyanin and allophycocyanin were obtained after Thermosynechococcus PCC 6715 biomass disintegration. The FF process with air flow of 2.4 L·h -1 resulted in purification factors up to 1.47 and partitioning coefficients of about 39, and did not require the addition of surfactants. A temperature of 35˚C allowed for the highest partitioning coefficient of 67.6 and yield of 76%; however, the purity of C-PC in condensate at this temperature was lower than at 25˚C. ATPE was tested in 20 different systems consisting of polyethylene glycol and phosphate or citrate salts, of which PEG1500-citrate gave the highest purification factor value of 2.31. Conversely, a partitioning coefficient of 2416 and 1094 were obtained for the PEG1500-phosphate and PEG3000-phosphate systems, respectively. Interestingly, the use of FF condensate in subsequent ATPE step resulted, for the first time, in the separation of the polymer phase into two fractions, one contained C-phycocyanin and the other allophycocyanin. It can be concluded that the use of a two-step system of FF and ATPE is a viable way to separate phycobiliproteins.},
 type={Article accepted},
 title={Separation and purification of phycobiliproteins from Thermosynechococcus PCC 6715 by foam fractionation and aqueous two-phase extraction},
 URL={http://ochroma.man.poznan.pl/Content/130414/e62_internet.pdf},
 doi={10.24425/cpe.2024.149457},
 keywords={phycobiliproteins, purification, aqueous two-phase extraction, foam fractionation},
}