@ARTICLE{Isah_Tasiu_IN_2013,
 author={Isah, Tasiu and Mujib, Abdul},
 volume={vol. 55},
 number={No 2},
 journal={Acta Biologica Cracoviensia s. Botanica},
 howpublished={online},
 year={2013},
 publisher={Biological Commission of the Polish Academy of Sciences – Cracow Branch},
 abstract={An efficient system for plant regeneration of Senna occidentalis from hypocotyl-derived callus was developed. Callus was induced from leaf and hypocotyl explants on MS medium amended with 9.04 μM 2,4-D + 2.22 μM BAP and 10.74 μM NAA + 2.22 μM BAP. Medium browning due to leaching of compounds from callus was encountered and ameliorated through incorporation of 2.84 μM ascorbic acid. Leaf-derived callus showed no shoot induction ability, while hypocotyl-derived callus produced shoots in all cytokinin-amended treatments and also in combination with 2.68 μM NAA. For shoot formation, BAP-augmented treatments were better than medium with Kin added. Rhizogenesis was better on 1/2 MS basal medium with IBA than in the NAA and IAA treatments. Regenerated plants were acclimatized with 94% survival and showed similar morphology to field-grown plants.},
 type={Artykuły / Articles},
 title={IN VITRO PLANT REGENERATION OF COFFEE SENNA (SENNA OCCIDENTALIS) FROM HYPOCOTYL-DERIVED CALLUS},
 URL={http://ochroma.man.poznan.pl/Content/81806/PDF/13_isah.pdf},
 doi={10.2478/abcsb-2013-0031},
 keywords={Senna occidentalis, callus, regeneration. shoot induction, rhizogenesis, acclimatization},
}