@ARTICLE{Ząbkiewicz_Anna_The_2014, author={Ząbkiewicz, Anna and Myga-Nowak, Magdalena and Bandurska, Katarzyna and Paczyńska, Justyna and Szybecka, Anna and Krupa, Piotr}, volume={vol. 40}, number={No 2}, journal={Archives of Environmental Protection}, howpublished={online}, year={2014}, publisher={Polish Academy of Sciences}, abstract={This study characterizes mycorrhiza helper bacteria (MHB) from selected unpolluted locations as well as subjected to industrial emissions. To determine the species of bacteria isolated from the roots of ectomycorrhizal pine and birch, a method based on the sequence analysis of a 16S rRNA gene was used. The isolated bacteria were initially characterized by available biochemical methods and phenotypic observation. On the selected bacteria representatives isolation of DNA was performed, on which the PCR reaction was carried out. In this way amplified samples were automatically sequenced and the obtained results were compared to public databases. Among the isolated bacteria Pseudomonas fluorescens SBW25 and Burkholderia xenovorans LB400 species were dominant.}, type={Artykuły / Articles}, title={The Application of PCR Reaction for Identification of MHB Bacteria Species}, URL={http://ochroma.man.poznan.pl/Content/85936/PDF/10265-Volume40-Issue2-paper_09.pdf}, doi={10.2478/aep-2014-0020}, keywords={mycorrhiza, helper bacteria (MHB), 16sRNA gene}, }