Nauki Biologiczne i Rolnicze

Journal of Plant Protection Research

Zawartość

Journal of Plant Protection Research | 2022 | vol. 62 | No 2

Abstrakt

Reports of weed resistance to herbicides have increased in recent years and differentiation in resistance mechanisms is considered to be a concern for the future of weed science. The aim of this work was to characterize the mechanisms of resistance to herbicides associated with Conyza sp. complex and analyze their implications. Aspects of the action of herbicides commonly used in their control will be addressed, in addition to a description of the mechanisms involved in multiple resistance in Conyza species.
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Autorzy i Afiliacje

Luisa Carolina Baccin
1
ORCID: ORCID
Alfredo Junior Paiola Albrecht
2
ORCID: ORCID
Leandro Paiola Albrecht
2
ORCID: ORCID
André Felipe Moreira Silva
3
ORCID: ORCID
Ricardo Victoria Filho
1
ORCID: ORCID

  1. Fitotecnia, Escola Superior de Agricultura Luiz de Queiroz, Piracicaba, Sãu Paulo, Brazil
  2. Departamento de Ciências Agronômicas, Universidade Federal do Paraná, Palotina, Brazil
  3. Crop Science Pesquisa e Consultoria Agronômica LTDA, Maripá, Paraná, Brazil

Abstrakt

The world population, and thus the need for food, is increasing every day. This leads to the ultimate question of how to increase food production with limited time and scarce land. Another obstacle to meet the food demand includes the stresses a plant goes through. These may be abiotic or biotic, but the majority are biotic, i.e., plant diseases. The major challenge is to mitigate plant diseases efficiently, more quickly and with less manpower. Recently, artificial intelligence has turned to new frontiers in smart agricultural science. One novel approach in plant science is to detect and diagnose plant disease through deep learning and hyperspectral imaging. This smart technique is very advantageous for monitoring large acres of field where the availability of manpower is a major drawback. Early identification of plant diseases can be achieved through machine learning approaches. Advanced machine learning not only detects diseases but also helps to discover gene regulatory networks and select the genomic sequence to develop resistance in crop species and to mark pathogen effectors. In this review, new advancements in plant science through machine learning approaches have been discussed.
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Autorzy i Afiliacje

Siddhartha Das
1
ORCID: ORCID
Sudeepta Pattanayak
2
ORCID: ORCID
Prateek Ranjan Behera
3

  1. Department of Plant Pathology, M.S. Swaminathan School of Agriculture, Centurion University of Technology and Management, Paralakhemundi, Odisha, India
  2. Division of Plant Pathology, ICAR – Indian Agricultural Research Institute, New Delhi, India
  3. Department of Plant Pathology, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar, India

Abstrakt

Surface water retention of leaves and fruits of apple ( Malus domestica Borkh.) and sweet cherry ( Prunus avium L.), was evaluated under controlled environmental conditions in order to determine the retention potential at different growth stages. Dipping and spraying, with and without non-ionic surfactant, were used as application systems. Water retention was expressed as the ratio between the weight difference of the organ before and post application and organ weight before application. Leaf water retention by dipping was 62 and 64% for ‘Royal Gala’ and ‘Fuji’ apples, respectively, and 37 and 50% by spraying. The surfactant tended to reduce foliar water retention by spraying on both species. An exponential reduction of fruit water retention was observed during their growth. Fruit dipping generated the highest water retention, with values of 50% at the earliest stage. Then, water retention stabilized at 1–2%, when the apples and sweet cherries diameter reached 25 and 15 mm, respectively, despite dipping or spraying. The surfactant tended to increase water retention at early fruit stages and to reduce it with fruit growth. These results can be useful for estimating the potential residue on leaves and fruits in apple and sweet cherry trees, in both the orchard (spraying) and the packing house (dipping).
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Autorzy i Afiliacje

José Antonio Yuri
1
ORCID: ORCID
Miguel Palma
1
Álvaro Sepúlveda
1
Mariana Moya
1

  1. Centro de Pomáceas, Facultad de Ciencias Agrarias, Universidad de Talca, Talca, Chile

Abstrakt

The main pathogen that deteriorates the quality of rose flowers during the postharvest stage belongs to the fungal genus Botrytis. The chemical products used to control the disease caused by this pathogen have been losing effectiveness due to the appearance of resistance. The present study describes the in vitro and in vivo fungicidal activity of Pelargonium graveolens essential oil and its chemical composition. The essential oil was obtained by hydrodistillation, and the in vitro fungicidal activity was determined by agar diffusion assays, showing 100% of fungal growth inhibition at 250 ppm. In vivo assays were performed on Rosa grandiflora flowers treated with 250 ppm of P. graveolens essential oil, using distillate water as a positive control and the commercial fungicide carbendazim as a negative one. No significant differences were obtained between the treatment with the essential oil and the treatment with the commercial fungicide. The chemical profile of the essential oil was determined by GC-MS. The main compounds detected were geraniol (24.89%), citronellol (19.50%), linalool (10.92%) and γ-eudesmol (8.93%). These results encourage the possible use of P. graveolens essential oil for the control of B. cinerea in rose flowers.
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Autorzy i Afiliacje

María Inés Stegmayer
1
ORCID: ORCID
Norma Hortensia Álvarez
1 2
ORCID: ORCID
Néstor Gaspar Sager
2
ORCID: ORCID
Marcela Alejandra Buyatti
2
ORCID: ORCID
Marcos Gabriel Derita
1 3
ORCID: ORCID

  1. Producción y Protección Vegetal, ICiAgro Litoral, UNL, CONICET, FCA, Argentina
  2. Cultivos Intensivos, Facultad de Ciencias Agrarias, Universidad Nacional del Litoral, Santa Fe, Argentina
  3. Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina

Abstrakt

The aim of the research was to analyze the degree of infection of winter wheat by fungal diseases and to evaluate the morphological and physiological parameters of plants depending on varied foliar fertilization (with and without the ionic form of silver) and applied plant protection agents (active ingredients: propiconazole, fenpropidin, azoxystrobin) in the 2016/2017, 2017/2018 and 2018/2019 growing seasons. The results showed that micronutrient fertilizers with silver and pesticides reduced the severity of fungal diseases better than the control. In most cases, foliar fertilizers enriched with the ionic form of silver at a dose of 1 and 2 l · ha −1 were the most effective. Moreover, foliar fertilization and pesticides had a positive effect on the morphology of wheat. Combined treatment (micronutrient fertilizer with silver and pesticide at a dose of 1 l · ha −1) increased stalk length and weight, ear weight and thousand grain weight to the greatest extent in comparison to the other treatments, while the pesticides stimulated ear length the most. In turn, microelement fertilizers with silver at a dose of 1 and 2 l · ha −1 were better in terms of flag leaf length. Wheat treated with foliar fertilizer and pesticide significantly improved the chlorophyll content based on the leaf greenness index (SPAD). It was found that the foliar application of microelements with silver is promising for use in agriculture because they controlled fungal diseases and ensured the good condition of plants more effectively than pesticides harmful to the environment.
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Autorzy i Afiliacje

Ewelina Matras
1
ORCID: ORCID
Anna Gorczyca
1
ORCID: ORCID
Marek Kołodziejczyk
2
ORCID: ORCID
Bogdan Kulig
2
ORCID: ORCID
Sebastian Wojciech Przemieniecki
3
ORCID: ORCID

  1. Department of Microbiology and Biomonitoring, University of Agriculture in Krakow, Krakow, Poland
  2. Department of Agroecology and Plant Production, University of Agriculture in Krakow, Krakow, Poland
  3. Department of Entomology, Phytopathology and Molecular Diagnostics, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

Abstrakt

A field trial on the transfer of pyrimethanil, cyprodinil and cyflufenamid residues from apple trees of Idared cultivar to hives by honeybees Apis mellifera was carried out. Two days after spraying (Faban 500 SC and Kendo 50 EW), and on the day of spraying (Chorus 50 WG), the quantities of residues on leaves and flowers of apple trees and pollen were as follows: pyrimethanil: 1.45 μg per cm2 of leaves, 11.51 μg per single flower and 7.18 μg · g −1 of pollen, cyprodinil:1.35, 8.64 and 7.94 μg, and cyflufenamid: 0.064, 0.266 and 0.11 μg, respectively. All of them subsequently disappeared exponentially. Two days after, and on the day of spraying, pyrimethanil (1.81 μg · g −1), cyprodinil (up to 0.55 μg · g −1) and cyflufenamid (0.04 μg · g −1) were found in worker bees. Residues of all used chemicals were found in the brood, honey and wax samples. The residues of pyrimethanil, cyprodinil and cyflufenamid in worker bees exceeded the level of 0.2% of the LD50, which indicates that their application rates (doses) are safe for the honey bee.
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Autorzy i Afiliacje

Bartosz Piechowicz
1 2
ORCID: ORCID
Aleksandra Kuliga
1
Damian Kobylarz
1
Anna Koziorowska
2 3
ORCID: ORCID
Lech Zaręba
4
ORCID: ORCID
Magdalena Podbielska
1
ORCID: ORCID
Iwona Piechowicz
5
Stanisław Sadło
6

  1. Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszów, Poland
  2. Interdisciplinary Center for Preclinical and Clinical Research, University of Rzeszów, Poland
  3. Institute of Material Engineering, College of Natural Sciences, University of Rzeszów, Poland
  4. Interdisciplinary Centre for Computational Modelling, College of Natural Sciences, University of Rzeszów, Poland
  5. Independent researcher, Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszów, Poland
  6. Professor retired, Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszów, Poland

Abstrakt

The red-banded stink bug, Piezodorus guildinii, is an important pest in soybean. Information on its distribution is needed to determine the most effective timing for pest control and strategies to avoid yield losses, such as adequate monitoring. The present study was aimed at examining the temporal variation and spatial distribution of P. guildinii in soybean. The experimental design comprised an area of 5400 m² planted with soybean, with 54 plots of 100 m² each, in which plants were examined weekly for nymphs and adults of P. guildinii with a beating sheet. Evaluations were carried out from soybean emergence to harvest; however, P. guildinii occurred only during the reproductive stage. Based on aggregation indices, theoretical frequency distributions, and semivariograms, nymphs and adults were randomly distributed at the beginning of infestation but, tended to be aggregate during pod setting and seed filling. Our findings have a contribution to improving pest sampling systems and infestation mapping, including future semiochemicals studies.
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Autorzy i Afiliacje

Rafael Azevedo Silva
1
Paulo Eduardo Degrande
2
ORCID: ORCID
Bruno Souza Martins
1
ORCID: ORCID
Ellen Patricia Souza
2
ORCID: ORCID
Marcos Gino Fernandes
2
ORCID: ORCID

  1. Department of Plant Protection, Federal Institute of Mato Grosso do Sul, Brazil
  2. Department of Agricultural Science, Federal University of Grande Dourados, Dourados, Brazil

Abstrakt

An efficient application of phytosanitary products depends, among other factors, on a good selection of nozzles and the application volume rate of the solution used. Thus, the objective of this work was to evaluate the efficiency of different models of hydraulic tips and application volume rates on spray coverage on targets positioned in the upper, middle and lower thirds of corn plants. The application volume rates evaluated were: 50 l · ha −1; 100 l · ha −1; 150 l · ha −1; 200 l · ha −1; 300 l · ha −1 and 400 l · ha −1. The following nozzles were used: TT 11001, TTJ60 11002, TXA 8003, 30HCX 12, GRD120 02 and GAT11002. Applications were carried out in phenological stages V6–V7 of corn plants. There was a directly proportional relationship between an increase in application volume rate and the levels of spray coverage and droplet density in the three thirds of corn plants. The application volume rate evaluated, except for 50 l · ha −1 in the lower third, provided a number of droplets compatible with the literature recommendations for the application of systemic fungicides. All tips evaluated provided a number of droplets compatible with the recommendations in the literature for the application of systemic fungicides, therefore, they can be recommended for use in spraying on corn crops.
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Autorzy i Afiliacje

Roxana Stefane Mendes Nascimento
1
ORCID: ORCID
Douglas Ferreira Parreira
2
Juliana Souza Milagres
3
Danilo Felipe Afonso
3
Pedro Luid de Sousa Oliveira
4
Rafael Guimarães Silva Moraes
4

  1. Fitotecnia, Universidade Federal de Viçosa, Viçosa, Brazil
  2. Fitopatologia, Universidade Federal de Viçosa, Viçosa, Brazil
  3. Agronomia, Universidade Federal de Viçosa, Viçosa, Brazil
  4. Agronomia, Universidade Estadual da Região Tocantina do Maranhão, Imperatriz, Brazil

Abstrakt

Strawberry leaves showing leaf blight symptoms were collected from six different farms in Ismailia and Beheira Governorates in Egypt during the 2020–2021 growing season. Eight bacterial isolates, i.e., Pa1, Pa2, Pa3, Pa4 (Ismailia farms) and Pa5, Pa6, Pa7 and Pa8 (Beheira farms) were isolated. A pathogenicity test of bacterial isolates was carried out using detached strawberry leaf technique. All bacterial isolates produced leaf blight disease symptoms. Isolates Pa2 and Pa6 showed the highest pathogenic characteristics with clear symptoms on detached strawberry leaves. The phenotypic, biochemical and physiological characters of the highest pathogenic isolates were confirmed by PCR analysis using 16S rRNA gene. The two bacterial isolates were identified as Pantoea ananatis with similarity of 97.05% with accession number MH_127816.1 (isolate Pa2, Ismailia), while the isolate ( Pa6, Beheira) with similarity of 97.03% with accession number NR_026045.1. The 16S rDNA sequences were deposited in the GenBank nucleotide databases under accession numbers OM258167 and OM279507, respectively. According to the pathogenicity test, morphological and physiological characteristics as well as molecular data (16S rRNA sequencing analysis), this finding is the first report of P. ananatis as a causal agent of strawberry leaf blight disease in Egypt.
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Autorzy i Afiliacje

Tarek Gomaa Abdel-Gaied
1
ORCID: ORCID
Hassan Abd-El-Khair
1
ORCID: ORCID
Maryan Makram Youssef
2
Shereen Abu El-Maaty
3
Maurice Sabry Mikhail
2

  1. Department of Plant Pathology, National Research Centre, Dokki, Giza, Egypt
  2. Department of Plant Pathology, Faculty of Agriculture, Cairo University, Giza, Egypt
  3. Department of Genetics, Faculty of Agriculture, Cairo University, Giza, Egypt

Abstrakt

Weeds in sweet corn reduce the yield and are economically more harmful than other pests. The aim of this study was to evaluate the effects of mechanical weed control and efficacy of pre- and postemergence applied herbicides in sweet corn, and their influence on weed control expressed by various indices, corncob yield and net return. Field studies were carried out with preemergence thiencarbazone-methyl + isoxaflutole (at 29.7 + 74.3 g · ha –1), postemergence S-metolachlor + terbuthylazine (937.5 + 562.5 g · ha –1), mesotrione + terbuthylazine (100 + 652 g · ha –1), terbuthylazine + mesotrione + S-metolachlor (656.3 + + 131.3 + 1093.8 g · ha –1), weed free (WF, hand weeding), and mechanical weeding (MW, hoeing) to assess weed control, corncob yield and net return. Variability in potential yield losses was observed between years due to weather conditions at the level of 30 to even 93%. Hand weeding was the most effective, but it is expensive and needs is labour consuming, unlike mechanical weeding which was the cheapest but simultaneously the least effective. Among pre- and postemergence applied herbicides, a mixture of terbuthylazine + mesotrione + S-metolachlor was the most efficacious weed control treatment. It gave high corncob yield and economic net return.
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Autorzy i Afiliacje

Robert Idziak
1
ORCID: ORCID
Hubert Waligóra
1
ORCID: ORCID
Violetta Szuba
1

  1. Department of Agronomy, Poznan University of Life Sciences, Poznan, Poland

Abstrakt

This paper reports a study on genetic identification of Parthenium hysterophorus and the ability of P. hysterophorus crude extract (PHCE) to inhibit germination of weeds in palm oil plantations, particularly slender buttonweed ( Diodia ocimifolia). Parthenium hysterophorus is a noxious plant with invasive properties. It has become one of the world’s seven most devastating and hazardous weed‑s due to its allelopathic properties. However, a comprehensive study on its genetic identification and its herbicidal activity against weeds in palm oil plantations had never been carried out. In the present study, internal transcribes spacer (ITS) region-based analysis was used as a molecular marker to ascertain the local Rumput Miang Mexico (RMM) as a P. hysterophorus species. From the results, the DNA sequence obtained was 99% matched with P. hysterophorus species database. In the dose-response bioassay study, the phytotoxicity of PHCE on D. ocimifolia was simulated under laboratory conditions. Methanolic extract of P. hysterophorus significantly inhibited germination and growth of shoots and radicles of D. ocimifolia at low concentrations (1−5 g ∙ l−1). A study on the herbicidal activity of PHCE could be an additional management approach for this phytotoxic species and an alternative way to naturally control weeds on palm oil plantations.
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Autorzy i Afiliacje

Noorul Jannah Zainuddin
1
ORCID: ORCID
Norazlinaliza Salim
2
Norhayu Asib
3
Siti Efliza Ashari
2
Dzolkhifli Omar
3
Gwendoline Cheng Lian
4

  1. Department of Chemical Technology and Food, Politeknik Tun Syed Nasir Syed Ismail, Johor, Malaysia
  2. Integrated Chemical Biophysics Research, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia
  3. Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, Malaysia
  4. Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia

Instrukcja dla autorów

Instructions for Authors

Manuscripts published in JPPR are free of charge. Only colour figures and photos are payed 61.5 € per one colour page JPPR publishes original research papers, short communications, critical reviews, and book reviews covering all areas of modern plant protection. Subjects include phytopathological virology, bacteriology, mycology and applied nematology and entomology as well as topics on protecting crop plants and stocks of crop products against diseases, viruses, weeds, etc. Submitted manuscripts should provide new facts or confirmatory data. All manuscripts should be written in high-quality English. Non-English native authors should seek appropriate help from English-writing professionals before submission. The manuscript should be submitted only via the JPPR Editorial System (http://www.editorialsystem.com/jppr). The authors must also remember to upload a scan of a completed License to Publish (point 4 and a handwritten signature are of particular importance). ALP form is available at the Editorial System. The day the manuscript reaches the editors for the first time is given upon publication as the date ‘received’ and the day the version, corrected by the authors is accepted by the reviewers, is given as the date ‘revised’. All papers are available free of charge at the Journal’s webpage (www.plantprotection.pl). However, colour figures and photos cost 61.5 € per one colour page.

General information for preparing a manuscript

All text should be written in a concise and integrated way, by focusing on major points, findings, breakthrough or discoveries, and their broad significance. All running text should be in Times New Roman 12, 1.5 spacing with all margins 2.5 cm on all sides.

Original article

The original research articles should contain the following sections: Title – the title should be unambiguous, understandable to specialists in other fields, and must reflect the contents of the paper. No abbreviations may be used in the title. Name(s) of author(s) with affiliations footnoted added only to the system, not visible in the manuscript (Double Blind Reviews). The names of the authors should be given in the following order: first name, second name initial, surname. Affiliations should contain: name of institution, faculty, department, street, city with zip code, and country. Abstract – information given in the title does not need to be repeated in the abstract. The abstract should be no longer than 300 words. It must contain the aim of the study, methods, results and conclusions. If used, abbreviations should be limited and must be explained when first used. Keywords – a maximum of 6, should cover the most specific terms found in the paper. They should describe the subject and results and must differ from words used in the title. Introduction – a brief review of relevant research (with references to the most important and recent publications) should lead to the clear formulation of the working hypothesis and aim of the study. It is recommended to indicate what is novel and important in the study. Materials and Methods – in this section the description of experimental procedures should be sufficient to allow replication. Organisms must be identified by scientific name, including authors. The International System of Units (SI) and their abbreviations should be used. Methods of statistical processing, including the software used, should also be listed in this section. Results – should be presented clearly and concisely without deducting and theori sing. Graphs should be preferred over tables to express quantitative data. Discussion – should contain an interpretation of the results ( without unnecessary repetition) and explain the influence of experimental factors or methods. It should describe how the results and their interpretation relate to the scientific hypothesis and/or aim of the study. The discussion should take into account the current state of knowledge and up-to-date literature. It should highlight the significance and novelty of the paper. It may also point to the next steps that will lead to a better understanding of the matters in question. Acknowledgements – of people, grants, funds, etc. should be placed in a separate section before the reference list. The names of funding organizations should be written in full. References In the text, papers with more than two authors should be cited by the last name of the first author, followed by et al. (et al. in italics), a space, and the year of publication (example: Smith et al. 2012). If the cited manuscript has two authors, the citation should include both last names, a space, and the publication year (example: Marconi and Johnston 2006). In the Reference section, a maximum of ten authors of the cited paper may be given. All references cited in the text must be listed in the Reference section alphabetically by the last names of the author(s) and then chronologically. The year of publication follows the authors’ names. All titles of the cited articles should be given in English. Please limit the citation of papers published in languages other than English. If necessary translate the title into English and provide information concerning the original language in brackets (e.g. in Spanish). The list of references should only include works from the last ten years that have had the greatest impact on the subject. Older references can be cited only if they are important for manuscript content. The full name of periodicals should be given. If possible, the DOI number should be added at the end of each reference. The following system for arranging references should be used: Journal articles Jorjani M., Heydari A., Zamanizadeh H.R., Rezaee S., Naraghi L., Zamzami P. 2012. Controlling sugar beet mortality disease by application of new bioformulations. Journal of Plant Protection Research 52 (3): 303-307. DOI: https://doi.org/10.2478/v10045-012-0049-9 Online articles Turner E., Jacobson D.J., Taylor J.W. 2011. Genetic architecture of a reinforced, postmating, reproductive isolation barrier between Neurospora species indicates evolution via natural selection. PLoS Genetics 7 (8): e1002204. DOI: https://doi.org/10.1371/journal.pgen.1002204 Books Bancrof J.D., Stevens A. 1996. Theory and Practice of Histological Techniques. 4th ed. Churchill Livingstone, Edinburgh, UK, 776 pp. Book chapters Pradhan S.K. 2000. Integrated pest management. p. 463-469. In: "IPM System in Agriculture. Cash Crop" (R.K. Upadhyaya, K.G. Mukerji, O.P. Dubey, eds.). Aditya Books Pvt. Ltd. New Delhi, India, 710 pp. Online documents Cartwright J. 2007. Big stars have weather too. IOP Publishing PhysicsWeb. Available on: https://doi.org/10.1371/journal.pgen.1002204

Tables, Figures, Phothographs, Drawings

Tables and figures should be uploaded as separated files at the submission stage. Their place in the manuscript should be clearly indicated by authors. Colour figures are accepted at no charge for the electronic version. In the hardcopy version of the journal, colour figures cost (65,5 € per one colour page). When attaching files please indicate if you want colour only in the online version or in both the online and the hardcopy. Photographs and RGB bitmaps should be provided in JPG or TIFF file format. They must have no less than 300 dpi resolution. The text column should be 8 cm wide and they must be at least 1000 pixels wide. Please send original (not resized) photograph(s), straight from a digital camera, without any text descriptions on the photo. Bitmaps combined with text object descriptions should be provided in MS Word or MS Powerpoint format. Text objects using Arial font-face should be editable (changing font-face or font size). Drawings should be provided in MS Word, MS Powerpoint, CorelDRAW or EPS file format and stored with original data file. Text objects using Arial font-face should be editable (changing font-face or font size). Charts (MS Excel graphs) should be provided in MS Excel file format, and stored with original MS Excel data file without captions but with the number of the figure attached. Please do not use bitmap fills for bar charts. Use colour fills only if necessary. Captions and legends should be added at the end of the text, referred to as "Fig." and numbered consecutively throughout the paper.

Rapid communications

Rapid communications should present brief observations which do not warrant the length of a full paper. However, they must present completed studies and follow the same scientific standards as original articles. Rapid communications should contain the following sections: Title Abstract - less than 300 words Key words - maximum 6 Text body Acknowledgements References The length of such submissions is limited to 1500 words for the text, one table, and one figure.

Reviews

Review articles are invited by the editors.Unsolicited reviews are also considered. The length is limited to 5000 words with no limitations on figures and tables and a maximum of 150 references. Mini-Review articles should be dedicated to "hot" topics and limited to 3000 words and a maximum two figures, two tables and 20 references.

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