Life Sciences and Agriculture

Journal of Plant Protection Research

Content

Journal of Plant Protection Research | 2023 | vol. 63 | No 4

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Abstract

The world population is projected to reach 9.8 billion in 2050, and 11.2 billion in 2100 (United Nations) and people will need food, and decrease the farming land. Thus, the importance of Internet of Things (IoT) and Computer Science (CS) in plant disease management are increasing now-a-days. Mobile apps, remote sensing, spectral signature analysis, artificial neural networks (ANN), and deep learning monitors are commonly used in plant disease and pest management. IoT improves crop yield by fostering new farming methods along with the improvement of monitoring and management through cloud computing. In the quest for effective plant disease control, the future lies in cutting-edge technologies. The integration of IoT, artificial intelligence, and data analytics revolutionizes monitoring and diagnosis, enabling timely and precise interventions. Cloud computing facilitates real-time data sharing and analysis empower farmers to combat diseases with unprecedented efficiency. By harnessing these innovations, agriculture can embrace sustainable practices and safeguard crop health, ensuring a bountiful and secure future for the global food supply.
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Authors and Affiliations

Suborna Rani
1
Kallol Das
2
ORCID: ORCID
F.M. Aminuzzaman
3
ORCID: ORCID
Benjamin Yaw Ayim
4
ORCID: ORCID
Natasza Borodynko-Filas
5
ORCID: ORCID

  1. Faculty of Computer Science and Engineering, Patuakhali Science and Technology University, Patuakhali, Bangladesh
  2. College of Agriculture and Life Sciences, Kyungpook National University, Daegu, Republic of Korea
  3. Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh
  4. Ministry of Food and Agriculture, Plant Protection and Regulatory Services Directorate, Ashanti, Ghana
  5. Plant Disease Clinic and Bank of Pathogens, Institute of Plant Protection – National Research Institute, Poznan, Poland
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Abstract

Seed endophytes are potential bioagents for plant protection and growth promoters. The question of the specifics of their isolation in cultural environments is not clear. The purpose of this study was to establish the nature of the interaction of endophytic fungi of wheat seeds with different levels of aggressiveness and presence in the mycobiota. Dual cultivation was carried out at potato-glucose agar (PGA), comparing with single fungal cultivation. The mutual influence of fungi during joint cultivation was established. Alternaria arborescens, which dominated in the mycobiota of wheat seeds from northeastern Ukraine, suppressed the development of only P enicillium. Nigrospora oryzae, Bipolaris sorokiniana, and Phoma developed faster than A. arborescens. Fusarium poae, and F. sporotrichioides competed for agar medium with N. oryzae. Known bioagents from wheat seeds showed unexpectedly low results. Trichothecium roseum formed a rejection zone during co-cultivation with F. graminearum. Trichoderma sp. Max18 (resistant to fludioxonil) on the 7th day inhibited the development of Penicillium, F. graminearum, and A. arborescens by 55, 48 and 26%, respectively. N. oryzae developed faster than the mycoparasitic fungus, but the latter began to parasitize it only from the 13th day.
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Authors and Affiliations

Tetiana Rozhkova
1 2
ORCID: ORCID
Liudmyla Biliavska
1
ORCID: ORCID
Yuriy Spychak
2
ORCID: ORCID

  1. Department of General and Soil Microbiology, Institute of Microbiology and Virology named after D.K. Zabolotny National, Academy of Sciences of Ukraine, Kyiv, Ukraine
  2. Department of Plant Protection, Sumy National Agrarian University, Sumy, Ukraine
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Abstract

The tomato crop is more susceptible to disease than any other vegetable, and it can be infected with over 200 diseases caused by different pathogens worldwide. Tomato plant diseases have become a challenge to food security globally. Currently, diagnosing and preventing tomato plant diseases is a challenge due to the lack of essential methods or tools. The traditional techniques of detecting plant disease are arduous and error-prone. Utilizing precise or automatic detection methods in spotting early plant disease can improve the quality of food production and reduce adverse effects. Deep learning has significantly increased the recognition accuracy of image classification and object detection systems in recent years. In this study, a 15-layer convolutional neural network is proposed as the backbone for single shot detector (SSD) to improve the detection of healthy, and three classes of tomato fruit diseases. The proposed model performance is compared with ResNet-50, AlexNet, VGG 16, and VGG19 as the backbone for Single shot detector. The findings of the experiment showed that the proposed CNN-SDD achieved 98.87% higher detection accuracy, which outperformed state-of-the-art models.
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Authors and Affiliations

Benedicta Nana Esi Nyarko
1
ORCID: ORCID
Wu Bin
1
Zhou Jinzhi
1
ORCID: ORCID
Justice Odoom
1
ORCID: ORCID

  1. School of Information Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, China
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Abstract

Snap bean production in Kenya is constrained by many pests and diseases, including the bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV). The occurrence of the dominant I gene in many snap bean cultivars has provided a measure of control over BCMV but the BCMNV overcomes this resistance. The current study aimed to screen a collection of locally grown snap bean commercial cultivars, landraces, breeding lines, and dry bean cultivars for the expression of resistance against BCMNV under both field and greenhouse conditions. The results showed that the evaluated snap bean cultivars were susceptible to BCMNV. The reactions of the genotypes to BCMNV varied from top, vein and local necrosis, mosaics, mottling, deformed leaves to stunted growth. Positive infection was confirmed through enzyme linked immunosorbent assays. The dry bean cultivars, which were used as resistant checks can be explored as sources of resistance to BCMNV in future breeding programs. Molecular analysis showed that the SW13 and elF4E markers were reliable in confirming the presence or absence of the dominant I gene and the recessive bc-3 gene, respectively. These molecular markers are useful in markerassisted breeding programs.
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Authors and Affiliations

Grace Wambui Watare
1
ORCID: ORCID
Bernard Mukiri Gichimu
1
ORCID: ORCID
Edith Esther Arunga
1
ORCID: ORCID

  1. Water and Agricultural Resource Management, University of Embu, Embu, Kenya
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Abstract

The present study was specifically designed to develop bio-fungicides that may help mitigate reliance on hazardous synthetic chemicals which give rise to environmental safety concerns. A survey (2021–2022) of local fruit markets in Islamabad, Pakistan included morpho-molecular disease identification. It revealed Penicillium chrysogenum as a major phytopathogenic fungi causing fruit rot. The fungicidal action of plant essential oils might be boosted by the technique of application therefore, nanoencapsulation of essential oil and chitosan was performed. To attain the objective of antimicrobial packaging, essential oil and chitosan tablets were encapsulated in spun bond sachets against P. chrysogenum using a sachet volatile phase technique. In vitro screening of nano encapsulated eucalyptus essential oil showed significant inhibition of radial growth of P. chrysogenum colonies at 1.6 mg · ml–1 followed by 1.4, 1.2 and 1.0 mg · ml –1. Results of Gas Chromatography and Mass Spectrometry revealed the presence of eucalyptol in eucalyptus EO as a major antifungal component. An in vivo experiment analyzing the efficacy of essential oil tablets against pre-inoculated peach fruit with P. chrysogenum at ambient temperatures (7–37°C) showed significant reduction in lesion diameter, disease severity and prolonged shelf-life of peaches of more than 2 weeks. The natural ripening process of peach was not affected by the presence of antifungal sachets as no significant alteration in weight loss of fruit was recorded. The suppressiveness of fungal mycelial growth of P. chrysogenum was directly proportional to increases in E. globulus oil concentration. This research may have a significant impact on prolonging the shelf-life of peach fruit.
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Authors and Affiliations

Khan Gull-e-laala
1
Gulshan Irshad
2
Farah Naz
2
Ashfaq Ahmed Hafiz
3

  1. Department of Plant Pathology, University of Poonch, Rawalakot, Pakistan
  2. Department of Plant Pathology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
  3. Department of Horticulture, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
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Abstract

The aim of this research was to prepare the basis for the certification of the apple orchard protection program by determining disappearance models for active ingredients (AIs) of plant protection products (PPPs) in fruits. Field trials were carried out in a conventional apple orchard protected with PPPs in accordance with the currently adopted program. Residues of their AIs were determined using Agilent GC-MS/MS 7000D and LC-MS/MS 6470 QQQ, and their decreases were expressed by the exponential formula: R t = R 0 × e–k × t. Of all the AIs found in mature fruits, captan disappeared at the fastest rate [t (1/2) in the range of 9 to 13 days], followed by fluopyram [t (1/2) = 13 days], tebuconazole [t (1/2) = 14 days] and carbendazim [t (1/2) in the range of 24 to 32 days]. With the exception of dithiocarbamates and some fungicides (e.g., Captan 80 WDG) based on captan and methyl thiophanate, other insecticides and fungicides currently recommended can be used up to 3 months before harvest practically with virtually no restrictions. From July 15 to August 15, the chemicals effective at application rates not exceeding 0.3 kg of AI per ha should be used. To protect apples against storage diseases, PPPs that are effective at a dose ≤ 0.1 kg AI per ha (e.g., certain triazoles or strobilurins) and applied not later than 1 month before harvest, should be used.
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Authors and Affiliations

Stanisław Sadło
1
Magdalena Szczepanik
2
Paweł Krawiec
3
Bartosz Piechowicz
4 5
ORCID: ORCID

  1. Institute of Biotechnology, College of Natural Sciences, University of Rzeszów, Rzeszów, Poland
  2. Bio Berry Polska sp. z o.o., Lublin, Poland
  3. Horti Team Paweł Krawiec, Lublin, Poland
  4. Institute of Biology, College of Natural Sciences, University of Rzeszów, Rzeszów, Poland
  5. Interdisciplinary Center for Preclinical and Clinical Research, University of Rzeszów, Werynia, Poland
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Abstract

Sorghum produces allelopathic compounds, including total phenolic compounds and sorgoleone, which exhibit a phytotoxic effect on weeds. The field study, carried out in 2016-2017, was designed as an one-factor experiment, in the randomized block design, in four replications, with Sucrosorgo 506, Rona 1, KWS Freya, KWS Juno, and KWS Sammos, to assess the impact of allelochemicals on weeds. Weed infestation was determined at the beginning of July. Individual weed species were collected from two random places in each plot and weighed. The aim of the laboratory study was to evaluate the total content of phenolic compounds, and sorgoleone in the early stages of plant development (5, 10, and 15 days after emergence) in varieties Rona 1, KWS Freya, KWS Juno, KWS Sammos, Farmsorgo 180, GK Aron, PR 845F, Sucrosorgo 506 and PR849F. The total content of phenolic compounds was determined using the colorimetric method, and the sorgoleone HPLC technique on a Flexar chromatographic set. The highest value of sorgoleone was observed in 15-day-old seedlings of KWS Juno, the lowest in 5-day-old seedlings of Sucrosorgo 506, the highest levels of total phenolic compounds in 5-day-old seedlings of PR 845F, the lowest in 15-day-old seedlings of Farmsorgo 180. The results do not fully confirm the beneficial effect of allelopathic compounds on reducing weed infestation, however, it is important to emphasize the diversity of cultivars used. The statistically insignificant results indicated that most varieties of sorghum plants do not exhibit a significant decrease in yield.
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Authors and Affiliations

Hubert Waligóra
1
ORCID: ORCID
Sylwiana Nowicka
1
Robert Idziak
1
ORCID: ORCID
Piotr Ochodzki
2
Piotr Szulc
1
Leszek Majchrzak
1

  1. Faculty of Agriculture, Horticulture and Bioengineering, Department of Agronomy, Poznań University of Life Sciences,Poznań, Poland
  2. Department of Applied Biology, Institute of Plant Breeding and Acclimatization – National Research Insitute in Radzikowo,Radzikowo, Poland
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Abstract

Weeds in sugar beet ( Beta vulgaris L.) can contribute to a significant reduction in the root yield. The species composition of these plants is an important factor influencing the competition with crops. The aim of the 2-year field experiment with mixtures of: phenmedipham + ethofumesate + metamitron + quinmerac; ethofumesate + metamitron + metamitron + + quinmerac + triflusulfuron-methyl; phenmedipham + ethofumesate + metamitron + + quinmerac + triflusulfuron-methyl + clopyralid + lenacil, and phenmedipham + ethofumesate + metamitron + quinmerac + triflusulfuron-methyl + clopyralid applied in split doses, microdoses and Conviso Smart technology was to determine the weed species community composition and effectiveness of weed control strategies. The most common species occurring in both years were: Chenopodium album L., Fallopia convolvulus L., and Geranium pusillum L. The communities had the highest values of biodiversity indices in 2020 and lower values in 2021. There were no statistically significant differences in the herbicidal effectiveness of the tested herbicidal technologies over both years of research and for individual main weed species – 95–99%. The use of all herbicide variants contributed to achieving significantly higher yields than untreated treatments, and contributed to an increase in profitability of cultivation, but this result depended on the selected strategy. The presented herbicide solutions were characterized by direct income at a similar level.
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Authors and Affiliations

Monika Grzanka
1
ORCID: ORCID
Łukasz Sobiech
1
ORCID: ORCID
Robert Idziak
1
ORCID: ORCID
Arkadiusz Filipczak
1
Grzegorz Skrzypczak
1
ORCID: ORCID

  1. Agronomy Department, Poznań University of Life Sciences, Faculty of Agriculture, Horticulture and Bioengineering,Poznań, Poland
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Abstract

Tea mosquito bugs (TMB; Helopeltis bradyi, Hemiptera, Miridae) are a main pest in tea and cacao plantations worldwide. Pheromone-mediated mating disruption (MD) is a promising strategy to manage TMB populations. Experiments were conducted to evaluate the simultaneous effect of TMB female delayed mating (1, 3 and 5 days delayed mating) and pairing duration (1 day, 5 days, and entire lifespan) on several reproductive parameters. Results showed that female delayed mating obviously affected egg number, egg viability, and offspring number, but male delayed mating did not show such effects. Shortened pairing durations had a significant effect on egg viability and offspring number but not on egg number. Increased delayed mating and pairing duration negatively affected adult longevity. In general, we noted that TMB reproductive performance is vulnerable to delayed mating and shortened pairing durations, thus providing essential guidance for the implementation of MD strategies of TMB in the field.
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Authors and Affiliations

Indriani Feberati Putri
1
Suputa Suputa
1
Alan Soffan
1
ORCID: ORCID

  1. Department of Plant Protection, Universitas Gadjah Mada, Yogyakarta, Indonesia
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Abstract

The population dynamics of Tetranychus urticae (two-spotted spider mite – TSSM) are influenced by environmental factors, which were investigated to identify methods limiting the development and harmfulness of this pest. This paper presents findings from a laboratory study on the effect of spraying with water-based extracts of Mentha piperita and Tagetes patula plants on the growth rate of TSSM larvae and nymphs. The time required for the transformation of the larvae into protonymphs was longer for T. patula water- based extracts than extracts from M. piperita. Differences in the time of transformation from the nymphal stage to adult TSSM were also observed depending on the plant waterbased extracts, their concentration, and the host plant on which nymphs were developing. The use of 100 g ∙ l-1 French marigold extract was associated with longer transformation time of nymphs on discs of pea leaves than all tested concentrations of French marigold extract applied to sugar beet leaves. Ten percent of the extracts from peppermint plants had a stronger limiting effect on TSSM nymphs on pea leaves than on bean or sugar beet leaves.
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Authors and Affiliations

Magdalena Jakubowska
1
ORCID: ORCID
Renata Dobosz
2
ORCID: ORCID
Alicja Szabelska-Beręsewicz
3
ORCID: ORCID
Joanna Zyprych-Walczak
3
ORCID: ORCID
Jolanta Kowalska
4
ORCID: ORCID
Anna Tratwal
1
ORCID: ORCID

  1. Department of Monitoring and Signalling of Agrophages, Institute of Plant Protection –National Research Institute, Poznań, Poland
  2. Department of Entomology and Animal Pests, Institute of Plant Protection – National Research Institute, Poznań, Poland
  3. Department of Mathematical and Statistical Methods, Poznań University of Life Sciences, Poznań, Poland
  4. Department of Organic Agriculture and Environmental Protection, Institute of Plant Protection – National Research Institute, Poznań, Poland

Authors and Affiliations

Mehmet Demir Kaya
1
ORCID: ORCID
Nurgül Ergin
2

  1. Department of Field Crops, Faculty of Agriculture, Eskişehir Osmangazi University, Eskişehir, Turkey
  2. Department of Field Crops, Faculty of Agriculture and Natural Sciences, Bilecik Şeyh Edebali University, Bilecik, Turkey
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Abstract

Lasiodiplodia theobromae and Pseudofusicoccum kimberleyense are pathogens causing trunk canker in Carya illioniensis and there are still no reports of effective forms of control. However, biological control is a promising measure. The objective of this work was to isolate, identify and evaluate the action of Trichoderma spp. in the in vitro control of L. theobromae and P. kimberleyense and to identify the compounds produced by the antagonist. Trichoderma spp. was identified by molecular technique and morphologically characterized. The antagonistic action of T. asperellum isolates (obtained from the rhizospheric soil, and of an isolate obtained from a commercial formulation) was evaluated by pairing cultures and volatile metabolites on L. theobromae and P. kimberleyense. Optimization of the cultivation method of T. asperellum was carried out and the compounds produced by the antagonist were identified by gas chromatography. Isolates obtained from the soil were identified as T. asperellum and decreased mycelial growth of L. theobromae and P. kimberleyense in the crop pairing test (48.98% S6 x Qt), as well as by volatile metabolites (29.85% SM21 x TR4). The cultivation conditions that generated the filtrates with the greatest antifungal action used 20 g · l –1 of corn maceration water, yeast extract 7.5 g · l –1, pH 5, agitation 100 rpm, sucrose 50 g · l –1, inoculum concentration 105 spores /ml. Among the identified compounds, some stood out for having bioactive action, such as pyran derivatives, celidoniol, deoxy, pentadecanoicacid, 2,3-dihydro-3,5-dihydroxy-6-methyl, propanoicacid, 1-methylethyl ester and 9-octadecenoic acid. The T. asperellum isolates showed potential for biocontrol in vitro, acting by different mechanisms.
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Authors and Affiliations

Jéssica Emília Rabuske
1
ORCID: ORCID
Marlove Fátima Brião Muniz
2
ORCID: ORCID
Thiarles Brun
1
ORCID: ORCID
Mateus Alves Saldanha
2
ORCID: ORCID
Janaina Silva Sarzi
2
ORCID: ORCID
Lucas Gracioli Savian
2
ORCID: ORCID
Clair Walker
2
ORCID: ORCID
Jéssica Mengue Rolim
3
ORCID: ORCID
Giovani Leone Zabot
4
ORCID: ORCID
Marcio Antonio Mazutti
5
ORCID: ORCID

  1. Universidade Regional Integrada do Alto Uruguai e das Missões, Frederico Westphalen, Rio Grande do Sul, Brazil
  2. Departamento de Defesa Fitossanitária, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil
  3. Departamento de Fitotecnia, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil
  4. Laboratório de Processos de Engenharia Agroindustrial, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil
  5. Departamento de Engenharia Química, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil

Instructions for authors

Instructions for Authors

Manuscripts published in JPPR are free of charge. Only colour figures and photos are payed 61.5 € per one colour page JPPR publishes original research papers, short communications, critical reviews, and book reviews covering all areas of modern plant protection. Subjects include phytopathological virology, bacteriology, mycology and applied nematology and entomology as well as topics on protecting crop plants and stocks of crop products against diseases, viruses, weeds, etc. Submitted manuscripts should provide new facts or confirmatory data. All manuscripts should be written in high-quality English. Non-English native authors should seek appropriate help from English-writing professionals before submission. The manuscript should be submitted only via the JPPR Editorial System (http://www.editorialsystem.com/jppr). The authors must also remember to upload a scan of a completed License to Publish (point 4 and a handwritten signature are of particular importance). ALP form is available at the Editorial System. The day the manuscript reaches the editors for the first time is given upon publication as the date ‘received’ and the day the version, corrected by the authors is accepted by the reviewers, is given as the date ‘revised’. All papers are available free of charge at the Journal’s webpage (www.plantprotection.pl). However, colour figures and photos cost 61.5 € per one colour page.

General information for preparing a manuscript

All text should be written in a concise and integrated way, by focusing on major points, findings, breakthrough or discoveries, and their broad significance. All running text should be in Times New Roman 12, 1.5 spacing with all margins 2.5 cm on all sides.

Original article

The original research articles should contain the following sections: Title – the title should be unambiguous, understandable to specialists in other fields, and must reflect the contents of the paper. No abbreviations may be used in the title. Name(s) of author(s) with affiliations footnoted added only to the system, not visible in the manuscript (Double Blind Reviews). The names of the authors should be given in the following order: first name, second name initial, surname. Affiliations should contain: name of institution, faculty, department, street, city with zip code, and country. Abstract – information given in the title does not need to be repeated in the abstract. The abstract should be no longer than 300 words. It must contain the aim of the study, methods, results and conclusions. If used, abbreviations should be limited and must be explained when first used. Keywords – a maximum of 6, should cover the most specific terms found in the paper. They should describe the subject and results and must differ from words used in the title. Introduction – a brief review of relevant research (with references to the most important and recent publications) should lead to the clear formulation of the working hypothesis and aim of the study. It is recommended to indicate what is novel and important in the study. Materials and Methods – in this section the description of experimental procedures should be sufficient to allow replication. Organisms must be identified by scientific name, including authors. The International System of Units (SI) and their abbreviations should be used. Methods of statistical processing, including the software used, should also be listed in this section. Results – should be presented clearly and concisely without deducting and theori sing. Graphs should be preferred over tables to express quantitative data. Discussion – should contain an interpretation of the results ( without unnecessary repetition) and explain the influence of experimental factors or methods. It should describe how the results and their interpretation relate to the scientific hypothesis and/or aim of the study. The discussion should take into account the current state of knowledge and up-to-date literature. It should highlight the significance and novelty of the paper. It may also point to the next steps that will lead to a better understanding of the matters in question. Acknowledgements – of people, grants, funds, etc. should be placed in a separate section before the reference list. The names of funding organizations should be written in full. References In the text, papers with more than two authors should be cited by the last name of the first author, followed by et al. (et al. in italics), a space, and the year of publication (example: Smith et al. 2012). If the cited manuscript has two authors, the citation should include both last names, a space, and the publication year (example: Marconi and Johnston 2006). In the Reference section, a maximum of ten authors of the cited paper may be given. All references cited in the text must be listed in the Reference section alphabetically by the last names of the author(s) and then chronologically. The year of publication follows the authors’ names. All titles of the cited articles should be given in English. Please limit the citation of papers published in languages other than English. If necessary translate the title into English and provide information concerning the original language in brackets (e.g. in Spanish). The list of references should only include works from the last ten years that have had the greatest impact on the subject. Older references can be cited only if they are important for manuscript content. The full name of periodicals should be given. If possible, the DOI number should be added at the end of each reference. The following system for arranging references should be used: Journal articles Jorjani M., Heydari A., Zamanizadeh H.R., Rezaee S., Naraghi L., Zamzami P. 2012. Controlling sugar beet mortality disease by application of new bioformulations. Journal of Plant Protection Research 52 (3): 303-307. DOI: https://doi.org/10.2478/v10045-012-0049-9 Online articles Turner E., Jacobson D.J., Taylor J.W. 2011. Genetic architecture of a reinforced, postmating, reproductive isolation barrier between Neurospora species indicates evolution via natural selection. PLoS Genetics 7 (8): e1002204. DOI: https://doi.org/10.1371/journal.pgen.1002204 Books Bancrof J.D., Stevens A. 1996. Theory and Practice of Histological Techniques. 4th ed. Churchill Livingstone, Edinburgh, UK, 776 pp. Book chapters Pradhan S.K. 2000. Integrated pest management. p. 463-469. In: "IPM System in Agriculture. Cash Crop" (R.K. Upadhyaya, K.G. Mukerji, O.P. Dubey, eds.). Aditya Books Pvt. Ltd. New Delhi, India, 710 pp. Online documents Cartwright J. 2007. Big stars have weather too. IOP Publishing PhysicsWeb. Available on: https://doi.org/10.1371/journal.pgen.1002204

Tables, Figures, Phothographs, Drawings

Tables and figures should be uploaded as separated files at the submission stage. Their place in the manuscript should be clearly indicated by authors. Colour figures are accepted at no charge for the electronic version. In the hardcopy version of the journal, colour figures cost (65,5 € per one colour page). When attaching files please indicate if you want colour only in the online version or in both the online and the hardcopy. Photographs and RGB bitmaps should be provided in JPG or TIFF file format. They must have no less than 300 dpi resolution. The text column should be 8 cm wide and they must be at least 1000 pixels wide. Please send original (not resized) photograph(s), straight from a digital camera, without any text descriptions on the photo. Bitmaps combined with text object descriptions should be provided in MS Word or MS Powerpoint format. Text objects using Arial font-face should be editable (changing font-face or font size). Drawings should be provided in MS Word, MS Powerpoint, CorelDRAW or EPS file format and stored with original data file. Text objects using Arial font-face should be editable (changing font-face or font size). Charts (MS Excel graphs) should be provided in MS Excel file format, and stored with original MS Excel data file without captions but with the number of the figure attached. Please do not use bitmap fills for bar charts. Use colour fills only if necessary. Captions and legends should be added at the end of the text, referred to as "Fig." and numbered consecutively throughout the paper.

Rapid communications

Rapid communications should present brief observations which do not warrant the length of a full paper. However, they must present completed studies and follow the same scientific standards as original articles. Rapid communications should contain the following sections: Title Abstract - less than 300 words Key words - maximum 6 Text body Acknowledgements References The length of such submissions is limited to 1500 words for the text, one table, and one figure.

Reviews

Review articles are invited by the editors.Unsolicited reviews are also considered. The length is limited to 5000 words with no limitations on figures and tables and a maximum of 150 references. Mini-Review articles should be dedicated to "hot" topics and limited to 3000 words and a maximum two figures, two tables and 20 references.

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