Life Sciences and Agriculture

Journal of Plant Protection Research

Content

Journal of Plant Protection Research | 2025 | vol. 65 | No 4

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Abstract

Compost piles serve as important habitats for various insect groups, including decomposers, predators, and parasitoids. While drosophilid fruit flies play a crucial role in organic matter recycling, the variation of their abundance and diversity in composters remains poorly understood. This study examines fruit fly assemblages across three compost localities in Poland: two orchards in Dąbrowice and Nowy Dwór-Parcela, and a vegetable-fruit farm in Skierniewice. Insects, collected using sweep netting and traps, varied in abundance. Dipteran flies were the most prevalent, representing 25 families, followed by beetles, bees, and wasps. Thrips and earwigs were the least represented. With 16 species from three genera, drosophilids were the predominant group, making up 95.6% of all specimens. These included seven cosmopolitan species, namely Drosophila melanogaster, D. hydei, D. immigrans, D. buskii, D. repleta, D. simulans, and Scaptomyza pallida, and three alien species, D. suzukii, Chymomyza amoena, and D. triauraria, the latter being a new Asian species recently reported in Poland and Europe. In addition to D. suzukii, another significant pest, Carpophilus hemipterus, was also recorded in the compost piles. Most species were fruit breeders or decaying plant material inhabitants, while others were mycetophagous (D. testacea, D. transversa, D. phalerata, D. funebris) or frugivorous-fungivorous (D. subobscura). Shannon-Wiener diversity indexes ranged between 1.1 and 1.4 across three localities, with the highest drosophilid diversity found at the Skierniewice farm. Drosophila melanogaster was the most numerous fruit fly at all the examined compost piles, while the relative abundance of other species depended on the composter site. These findings emphasize composters as underexplored hotspots for drosophilids, directing further study of their ecological niches and the potential presence of pest species.
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Authors and Affiliations

Katarzyna Michalska
1
Muhammad Arslan Ibrahim
1
Mariusz Martyka
1
Marcin Studnicki
2
Grażyna Soika
3

  1. Department of Plant Protection, Institute of Horticultural Sciences, Warsaw University of Life Sciences, Warsaw, Poland
  2. Department of Biometry, Institute of Agriculture, Warsaw University of Life Sciences, Warsaw, Poland
  3. Department of Plant Protection, The National Institute of Horticultural Research, Skierniewice, Poland
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Abstract

Beet moth (Scrobipalpa ocellatella) is a major pest of sugar beet crops in Europe, North Africa and Asia. Since its first detection in Poland a few years ago S. ocellatella has spread rapidly, causing extensive damage and is now one of the most serious threats to sugar beet. This problem is linked to climate change, high pest fecundity, its polyvoltinism and ini­tially almost latent feeding. Scrobipalpa ocellatella causes crop losses directly by feeding on plants and indirectly by creating favorable conditions for the growth of pathogens causing rot diseases. Heavy contamination of the beet crown with larval excrement creates ideal conditions for secondary fungal and bacterial infections. Sugar beets with severe root rot delivered to sugar plants are in many cases unsuitable for processing.

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Authors and Affiliations

Agnieszka Kamila Ulatowska
1
ORCID: ORCID
Dariusz Górski
1
ORCID: ORCID
Paweł Krystian Bereś
2
ORCID: ORCID

  1. Regional Experimental Station in Toruń, Institute of Plant Protection - National Research Institute, Toruń, Poland
  2. Regional Experimental Station in Rzeszów, Institute of Plant Protection - National Research Institute, Rzeszów, Poland
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Abstract

The present research reports the first record in Poland of the alien invasive and quarantine species Chaetosiphon (Pentatrichopus) fragaefolii (Cockerell, 1901) – the strawberry aphid. Native to North America, it is a critical pest and viral vector associated with strawberry crops. This study provides valuable insights into the genetic and morphological characteristics of the strawberry aphid. By integrating morphological and molecular analyses, the taxonomic resolution has been improved and the understanding of this economically important species has been deepened, particularly regarding its genetic diversity, distribution, and potential invasion routes. For the first time, scanning electron microscopy (SEM) analyses were performed to elucidate the general morphology, chaetotaxy, and sensilla of the antennae and mouthparts, contributing to improved knowledge related to both natural and chemical control strategies.
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Authors and Affiliations

Mariusz Kanturski
1
Minho Lee
2
Andżela Glumac
1
Seunghwan Lee
2

  1. Faculty of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, Katowice, Poland
  2. Insect Biosystematics Laboratory, Department of Agricultural Biotechnology, Research Institute of Agricultural and Life Sciences, Seoul National University, Seoul, Korea (South)
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Abstract

Crop yields depend not only on genetic traits, agronomic practices, and weather but also on effective crop protection. European agriculture aims to reduce the use of harmful chemicals while maintaining yields. Studies show that adjuvants can reduce the required doses of plant protection products. While their role in herbicide and fungicide applications is well documented, research on plant growth regulators remains limited. Field trials were conducted at the Institute of Plant Protection – National Research Institute in Poland to evaluate the impact of reduced doses of growth regulators, along with adjuvants, on the growth and yield of winter wheat. The study aimed to evaluate the potential and effectiveness of combining the plant growth regulators mepiquat chloride and prohexadione calcium with various adjuvants and additives in winter wheat under field conditions. The experimental treatments included a mixture of mepiquat chloride with prohexadione calcium (Medax Top 350 SC) applied together with citric acid; fertilizers such as urea and ammonium sulfate; and adjuvants including heptamethyltrisiloxane-modified polyalkylene oxide (Slippa), 76% paraffin oil (Atpolan 80 EC), 80% rapeseed oil fatty acid methyl esters, surface-active agents (surfactants), and a pH buffer (Atpolan BIO 80 EC), as well as ammonium sulfate ((NH4)2SO4 – 40%), a cationic surfactant (20%), and triethanolamine (5%) (AS 500 SL). The studies showed that it is possible to achieve the same results using half the standard doses of regulators, combined with adjuvants, as when using full doses. This also applied to plant height as well as qualitative and quantitative grain parameters. Additionally, the findings demonstrated that the effect of mepiquat chloride combined with prohexadione calcium and adjuvants varied depending on weather conditions during crop growth.
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Authors and Affiliations

Kinga Matysiak
1
ORCID: ORCID
Wojciech Miziniak
2
ORCID: ORCID
Jan Bocianowski
3
ORCID: ORCID
Jolanta Kowalska
4
ORCID: ORCID

  1. Department of Weed Science and Plant Protection Technique, Institute of Plant Protection – National Research Institute, Poznań, Poland
  2. Regional Experimental Station in Toruń, Institute of Plant Protection – National Research Institute, Toruń, Poland
  3. Department of Mathematical and Statistical Methods, Poznań University of Life Sciences, Poznań, Poland
  4. Department of Organic Farming and Environmental Protection, Institute of Plant Protection – National Research Institute, Poznań, Poland
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Abstract

Essential oils (EOs), from plants’ secondary metabolism, present bioactive compounds that may have insecticidal activity. Their use associated with parasitoid hymenoptera can be an alternative, however, little is known about the impact of EOs on the diamondback moth, Plutella xylostella and parasitoids. This work aimed to evaluate the effect of oils from Eucalyptus citriodora (EOEC) and Schinus terebinthifolius (EOST) (0.5% and 1%) on eggs and larvae of P. xylostella and on Trichogramma pretiosum. P. xylostella eggs were immersed in solutions containing EOs and in water (control). Treated cabbage leaf discs were offered to larvae. Eggs with EOs were offered to T. pretiosum females to evaluate parasitism and emergence. Already parasitized P. xylostella eggs were immersed in the treatments to observe the effect on the emergence of parasitoids. The survival of T. pretiosum adults exposed to EOs was recorded. The average number of inviable eggs was higher in treatments with EOs, ranging from 6.9 ± 1.31 to 13.0 ± 1.12 than in the control (3.5 ± 0.32) (p < 0.05). The average number of dead caterpillars was higher in treatments with EOs, ranging from 1.4 ± 0.24 to 2.2 ± 0.19 than in the control (0.1 ± 0.06) (p < 0.05) Leaf consumption in 48 h did not differ between the EOST treatment (0.05) (5.42%) and the control (9.7%). For the other treatments it was significantly lower: 3.8% (EOEC 0.5), 1.6 (EOEC 1), and 3.4 (EOST 1). Eggs treated with EOs prior to exposure to the parasitoid had lower parasitism rates than eggs that were already parasitized and subsequently treated with EOs. The mortality of adults exposed to EOs was 3% to 6%. The EOs of E. citriodora and S. terebinthifolius were toxic to eggs and larvae of P. xylostella, acted as feeding deterrents, and had minimal impact on parasitoids. As a result, they are promising for use in IPM.
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Authors and Affiliations

Júlia Pétra dos Santos Souza
1
ORCID: ORCID
Simone Mundstock Jahnke
1
ORCID: ORCID
Josué Sant’Ana
1
ORCID: ORCID

  1. Faculdade de Agronomia, Departamento de Fitossanidade, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
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Abstract

Due to their extensive cultivation and the significant impact of pests causing economically relevant losses, winter cereal crops require effective chemical protection. A major challenge in their protection is the steadily diminishing range of available plant protection products (PPPs), specifically the active substances (AS) they contain. Although the core principles of the European Green Deal have been temporarily suspended, the European Commission has continued to phase out several active substances. As a result, dozens of these substances have been withdrawn from the market in recent years, creating increasing difficulties in crop protection - particularly for winter cereals. This is partly due to the growing resistance of pests, stemming from reduced opportunities for rotating products with different mechanisms of action. Further reductions in active substances, in the absence of viable alternative methods for agrophage control, may potentially lead to a decrease in both the cultivated area and overall production volumes for economic reasons. Moreover, such a scenario increases the risk of illegal imports or off-label use of plant protection products (PPP).
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Authors and Affiliations

Przemysław Strażyński
1
ORCID: ORCID
Roman Kierzek
2
ORCID: ORCID
Marek Mrówczyński
1

  1. Department of Entomology and Agricultural Pests, Institute of Plant Protection – National Research Institute, Poznań, Poland
  2. Department of Weed Science and Plant Protection Techniques, Institute of Plant Protection – National Research Institute, Poznań, Poland
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Abstract

The use of fungicides to limit the development of soybean mycoses in modern agrocenoses is a common measure. At the same time, the use of symbiotic and phosphate-mobilizing bacteria increases the productivity and quality of the crop. The combination of biological agents and fungicides can have different effects and requires additional research. This study aimed to investigate the rhizosphere microbiome, mycosis resistance and soybean productivity under combined seed treatment with bacterial inoculants and fungicides. Pre-sowing treatment of soybean seeds was carried out with fungicides Maxim Star 025 FS or Kinto Duo, and 12 hours later the seeds were inoculated with the complex bioformulation Ecovital based on Bradyrhizobium japonicum and Bacillus megaterium. The effect of the integrated seed treatment on the rhizosphere microbiome (by high-throughput sequencing), the level of mycosis development and soybean productivity was determined. Under the combined use of biological and chemical preparations, the biodiversity of the rhizosphere microbiome improved, the relative abundance of fungicide-resistant phyla increased, and the number of sensitive taxa decreased. The low abundance of native rhizobia populations indicated the need for pre-sowing inoculation of soybean seeds. The combined use of fungicides and inoculation of seeds with Ecovital contributed to improved effectiveness of plant protection against ascochitosis and septoria and to an increase in soybean yield.
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Authors and Affiliations

Galyna Iutynska
1
Liudmyla Tytova
1
Svitlana Vozniuk
1
Nadiia Shevchuk
1
Liudmyla Bilyavska
1

  1. Department of General and Soil Microbiology, Zabolotny Institute of Microbiology and Virology of NASU, Kyiv, Ukraine
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Abstract

Environmental changes significantly impact the populations of various game mammal spe­cies, altering their biology, behavior, feeding preferences, and the extent of damage they cause to agricultural crops. This study focused on the current status of the wild boar (Sus scrofa L.) — a species that has become a major agricultural pest in certain agricultural crops. In Greater Poland, which is an agricultural region of Poland, wild boars cause the greatest damage to rapeseed and maize crops. Both plant species are mainly damaged during the spring and summer growing seasons, as wild boars not only forage in these fields but also create shelter areas within them, which leads to significant yield losses. This analysis considered both the negative consequences of its presence and the positive role it plays in the ecosystem. A substantial part of the presented information is based on research and observations conducted at the Game Breeding Center of the Institute of Plant Pro­tection – National Research Institute in Poznań (OHZ IOR-PIB), within hunting district no. 311 (Jarocin Forest District) near Winna Góra. This district covers areas within the Środa Wielkopolska and Miłosław municipalities.

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Authors and Affiliations

Pawel Wegorek
1
ORCID: ORCID
Weronika Herka
2
Filip Dolata
3

  1. Entomology and Animal Agrophagues, Institute of Plant Protection - NRI, Poznan, Poland
  2. Plant Health Surveillance Department, Provincial Inspectorate of Plant Health and Seed Inspection in Poznan, Sroda Wielkopolska, Poland
  3. Plant Health Surveillance Department, Provincial Inspectorate of Plant Health and Seed Inspection in Poznan, Poznan, Poland
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Abstract

Bacterial blight, caused by Xanthomonas campestris is a major disease of hazelnut (Corylus avellana) worldwide. Georgia is a significant hazelnut producer, but the presence of X. campestris has not been previously confirmed. In 2022–2023, hazelnut plants in nurseries across western Georgia were observed with symptoms of bacterial blight. Samples, including 538 symptomatic samples of both ‘Anakliuri’ (Georgian) and ‘Tonda di Giffoni’ (Italian) hazelnut cultivars, were collected from the Samegrelo and Guria regions in Georgia. DAS-ELISA with polyclonal antibodies identified 34 potentially positive for X. campestris. Additionally, samples were cultured on YPGA and King’s medium B for presumptive X. campestris identification based on yellow colonies with subsequent confirmation of 28 samples by a TaqMan triplex real-time PCR assay using species-specific primers. Sanger sequencing of the 16S rRNA gene, performed on 24 of these, with further BLAST analysis, revealed four isolates as X. campestris (GenBank Accession Numbers: PP437082.1, PP434581.1, PP434578.1, PP434556.1) showing up to 100% nucleotide identity to X. campestris pv. campestris strains isolated from different countries (India, Serbia, and Mexico) and sources. Pathogenicity was confirmed by inoculating young shoots (20–30 cm long) of 2-year-old potted hazelnut plants (‘Hall’s Giant’) with a bacterial suspension (108 CFU · ml–1) of each isolate. Bacterial cultures were re-isolated, fulfilling Koch’s postulates. This represents the first report of X. campestris in Georgia, highlighting the need for disease management strategies and the use of disease-free planting material to protect this economically important crop.
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Authors and Affiliations

Iveta Megrelishvili
1
Tinatin Elbakidze
2
Stefaan Werbrouck
3
Nino Maziashvili
1
Zurab Khidesheli
1
Amaal Rabaaoui
3
Levan Ujmajuridze
1

  1. Integrated Plant Protection Research, LEPL Scientific Research Center of Agriculture, Tbilisi, Georgia
  2. School of Science and Technology, University of Georgia, Tbilisi, Georgia
  3. Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium
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Abstract

The rice striped stem borer (Chilo suppressalis) is a significant pest of rice fields. This pest is mainly controlled using chemical methods. In this research, the various pest control tech­niques including solution pesticide spraying (Fipronil SC 5%, l · ha–1 or 50 g · ha–1 active sub­stance) and granule spreading (Fipronil G 0.2%, 20 kg · ha–1 or 40g · ha–1 active substances) were evaluated. The study included the following treatments: solution pesticide spraying with a UAV sprayer, spraying with a knapsack mist blower (KMB) sprayer, spraying with a knapsack motorized lance (KML) sprayer, hand granule spreading (HGS), granule spreading with a UAV granule spreader, and control (no application). The treatments were carried out in a randomized complete block design (RCBD) with four replications. The results indicated that the average consumed solution volume was 207.3, 195.6, and 18.2 l · ha–1 for KML, KMB, and UAV sprayers, respectively. The UAV sprayer had the lowest drift at 13%, while the KML sprayer had the highest at 51.57%. The yield of granule pesti­cide spreading was found to be higher than that of solution pesticide spraying. The granule spraying methods were more efficient than solution pesticide spraying in controlling the pest. The efficiency of HGS and UAV granule spreader methods was 87.25% and 79.7%, re­spectively, while the efficiency of the UAV, KMB, and KML sprayers was 72.7%, 79.9%, and 77.8%, respectively. The results also showed that the granule-spreading methods were more effective in controlling live larvae than solution pesticide spraying. In terms of income in­crease, the UAV granule spreader was found to be more profitable than the UAV sprayer, KMB sprayer, and KML sprayer, with increases of $109.2, $112.6, and $83.5 per hectare, respectively. The study recommends the use of the UAV granule spreader for controlling rice striped stem borers based on technical and economic evaluations of different pesticide application methods.

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Authors and Affiliations

Mohamoud Safari
1
ORCID: ORCID
Farzad Majidi Shilsar
2
ORCID: ORCID
Aziz Sheikhigarjan
3
ORCID: ORCID
Nikrooz Bagheri
1
ORCID: ORCID

  1. Department of Research on Agricultural Machinery and Mechanization, Agricultural Engineering Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
  2. Rice Research Institute of Iran (RRII), Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran
  3. Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
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Abstract

Cacao (Theobroma cacao) is significantly impacted each year by Frosty Pod Rot (FPR) caused by Moniliophthora roreri and Black Pod Rot (BPR) caused by Phytophthora species. The losses from these diseases pose a severe threat to cacao production worldwide. Consequently, cacao breeding programs focus on developing new clones that demonstrate high productivity potential and disease resistance. However, achieving this goal is challenging due to the lengthy selection process, the influence of environmental conditions on disease severity, and the need to avoid chemical control methods. Genetic resistance is, therefore, the most viable option for selecting and introducing new cacao clones to farmers. In this study, 40 cacao clones were evaluated from 2013 to 2017, with 20 clones sourced from the “Centro Agronómico Tropical de Investigación y Enseñanza” (CATIE) breeding program and 20 from the Fundación Hondureña de Investigación Agrícola (FHIA) breeding program. Three criteria were employed for clone selection: yield, percentage of diseased pods (PDP), and disease and production index (DPI). The results indicated that, depending on the objectives of the breeding program, these criteria can effectively be used to select new cacao clones that are both highly productive and disease-resistant. It was noted that cacao clones with high productivity are not always the most resistant to diseases, and vice versa. However, by combining these criteria, it is possible to identify cacao clones exhibiting high productivity potential and resistance to FPR and BPR.
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Authors and Affiliations

Marlon Enrique Lopez Torres
1
ORCID: ORCID
Oscar Arnulfo Ramirez-Argueta
1
Aroldo Dubon Dubon
1
Francisco Javier Diaz Matute
1

  1. Cacao and Agroforestry Program, Honduran Foundation for Agricultural Research (FHIA), La Lima Cortes, Honduras, 2067
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Abstract

In February 2022, preliminary field surveys were conducted to characterize patterns of abundance in the beneficial insect populations associated with fall armyworm (FAW) egg masses in selected Philippines corn fields. It was found that approximately 47.64% of the field-collected FAW egg masses were naturally parasitized by hymenopteran egg parasitoids. The most abundant, and possibly a habitual egg parasitoid of FAW, was Telenomus remus Nixon. The other egg parasitoids, Te. nawai Ashmead and Trichogramma chilonis, were likely only incidental parasitoids. This was based on the data gathered for population density, daily emergence patterns, local occurrence, and parasitism rates of these parasitoids. On the other hand, the remaining 52.36% of these egg masses successfully hatched and survived into the first instars. Further, the generalist predator Euborellia annulata was collected from soil samples in corn fields but with low patterns of abundance, and a possible explanation for this observation is provided. Overall, Te. remus and E. annulata are potential biocontrol agents and can be further evaluated for their ability to cause significant mortality on the first-generation FAW egg masses and the 2nd instars in corn plants. Future studies are recommended to establish their host and prey relationships in local corn agricultural landscapes. They are discussed in the context of developing conservation biocontrol (CoBC) tactics for preventing the establishment of FAW populations early in the cropping season.
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Authors and Affiliations

Marie Jou B. Beltran
1
ORCID: ORCID
Jessamyn Adorada
2
Ella Mae Lacanilao
1
Xiaodan Huang
3
Zhenying Wang
3

  1. National Crop Protection Center, University of the Philippines Los Baños, Agricultural Life Sciences Complex, Los Banos, Philippines
  2. Institute of Weed Science, Entomology and Plant Pathology, University of the Philippines Los Baños, LB Uichanco Hall, Los Banos, Philippines
  3. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Haidian District, Beijing, China
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Abstract

Plants have been associated with microflora for more than 400 million years, and this long-standing interconnection takes place in different regions of the plants. The complex community of microbes can be beneficial or pose a threat as pathogens. Previous studies have investigated plant immune responses and interactions with diverse microbes however, several aspects remain unclear and require further exploration. Plants utilize several small molecules through their associated biochemical pathways to aid this recognition process. This review examines recent progress on the distinct signaling pathways of various plant small molecules, including amino acids, lipids, and plant secondary metabolites, as well as the receptor-like kinases engaged in recruiting and scrutinizing the microbes involved in interactions. This review additionally explores how the rhizosphere and phyllosphere interact to shape the microbiome, facilitating plant homeostasis. Furthermore, it highlights the remarkable similarities between markets in human societies and those found in microbe- plant biological systems. Together, these ideas offer a framework for understanding how plants interact with advantageous microorganisms while simultaneously limiting harmful pathogens. The specific biochemical mechanisms and their interconnections are gradually being elucidated, providing a potential foundation for the development of novel plant protection strategies. With a holistic approach, these plant-microbe interactions can be exploited to engineer plants with multiomics approaches for improved performance, which can enhance crop resilience, promote sustainable agriculture, and address global food security in the long term.
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Authors and Affiliations

Gopalakrishnan Chellappan
1
ORCID: ORCID
Sree Gayathri E
1
ORCID: ORCID
Karthikeyan Gandhi
1
ORCID: ORCID
Senthil Alagarsamy
2
ORCID: ORCID
Saraswathi Thiruvenkatasamy
3
ORCID: ORCID
Kokiladevi Eswaran
4
ORCID: ORCID

  1. Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, India
  2. Department of Plant Physiology, Tamil Nadu Agricultural University, Coimbatore, India
  3. Department of Medicinal and Aromatic Crops, Tamil Nadu Agricultural University, Coimbatore, India
  4. Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India

Instructions for authors

Instructions for Authors

Manuscripts published in JPPR are free of charge. Only colour figures and photos are payed 61.5 € per one colour page JPPR publishes original research papers, short communications, critical reviews, and book reviews covering all areas of modern plant protection. Subjects include phytopathological virology, bacteriology, mycology and applied nematology and entomology as well as topics on protecting crop plants and stocks of crop products against diseases, viruses, weeds, etc. Submitted manuscripts should provide new facts or confirmatory data. All manuscripts should be written in high-quality English. Non-English native authors should seek appropriate help from English-writing professionals before submission. The manuscript should be submitted only via the JPPR Editorial System (http://www.editorialsystem.com/jppr). The authors must also remember to upload a scan of a completed License to Publish (point 4 and a handwritten signature are of particular importance). ALP form is available at the Editorial System. The day the manuscript reaches the editors for the first time is given upon publication as the date ‘received’ and the day the version, corrected by the authors is accepted by the reviewers, is given as the date ‘revised’. All papers are available free of charge at the Journal’s webpage (www.plantprotection.pl). However, colour figures and photos cost 61.5 € per one colour page.

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All text should be written in a concise and integrated way, by focusing on major points, findings, breakthrough or discoveries, and their broad significance. All running text should be in Times New Roman 12, 1.5 spacing with all margins 2.5 cm on all sides.

Original article

The original research articles should contain the following sections: Title – the title should be unambiguous, understandable to specialists in other fields, and must reflect the contents of the paper. No abbreviations may be used in the title. Name(s) of author(s) with affiliations footnoted added only to the system, not visible in the manuscript (Double Blind Reviews). The names of the authors should be given in the following order: first name, second name initial, surname. Affiliations should contain: name of institution, faculty, department, street, city with zip code, and country. Abstract – information given in the title does not need to be repeated in the abstract. The abstract should be no longer than 300 words. It must contain the aim of the study, methods, results and conclusions. If used, abbreviations should be limited and must be explained when first used. Keywords – a maximum of 6, should cover the most specific terms found in the paper. They should describe the subject and results and must differ from words used in the title. Introduction – a brief review of relevant research (with references to the most important and recent publications) should lead to the clear formulation of the working hypothesis and aim of the study. It is recommended to indicate what is novel and important in the study. Materials and Methods – in this section the description of experimental procedures should be sufficient to allow replication. Organisms must be identified by scientific name, including authors. The International System of Units (SI) and their abbreviations should be used. Methods of statistical processing, including the software used, should also be listed in this section. Results – should be presented clearly and concisely without deducting and theori sing. Graphs should be preferred over tables to express quantitative data. Discussion – should contain an interpretation of the results ( without unnecessary repetition) and explain the influence of experimental factors or methods. It should describe how the results and their interpretation relate to the scientific hypothesis and/or aim of the study. The discussion should take into account the current state of knowledge and up-to-date literature. It should highlight the significance and novelty of the paper. It may also point to the next steps that will lead to a better understanding of the matters in question. Acknowledgements – of people, grants, funds, etc. should be placed in a separate section before the reference list. The names of funding organizations should be written in full. References In the text, papers with more than two authors should be cited by the last name of the first author, followed by et al. (et al. in italics), a space, and the year of publication (example: Smith et al. 2012). If the cited manuscript has two authors, the citation should include both last names, a space, and the publication year (example: Marconi and Johnston 2006). In the Reference section, a maximum of ten authors of the cited paper may be given. All references cited in the text must be listed in the Reference section alphabetically by the last names of the author(s) and then chronologically. The year of publication follows the authors’ names. All titles of the cited articles should be given in English. Please limit the citation of papers published in languages other than English. If necessary translate the title into English and provide information concerning the original language in brackets (e.g. in Spanish). The list of references should only include works from the last ten years that have had the greatest impact on the subject. Older references can be cited only if they are important for manuscript content. The full name of periodicals should be given. If possible, the DOI number should be added at the end of each reference. The following system for arranging references should be used: Journal articles Jorjani M., Heydari A., Zamanizadeh H.R., Rezaee S., Naraghi L., Zamzami P. 2012. Controlling sugar beet mortality disease by application of new bioformulations. Journal of Plant Protection Research 52 (3): 303-307. DOI: https://doi.org/10.2478/v10045-012-0049-9 Online articles Turner E., Jacobson D.J., Taylor J.W. 2011. Genetic architecture of a reinforced, postmating, reproductive isolation barrier between Neurospora species indicates evolution via natural selection. PLoS Genetics 7 (8): e1002204. DOI: https://doi.org/10.1371/journal.pgen.1002204 Books Bancrof J.D., Stevens A. 1996. Theory and Practice of Histological Techniques. 4th ed. Churchill Livingstone, Edinburgh, UK, 776 pp. Book chapters Pradhan S.K. 2000. Integrated pest management. p. 463-469. In: "IPM System in Agriculture. Cash Crop" (R.K. Upadhyaya, K.G. Mukerji, O.P. Dubey, eds.). Aditya Books Pvt. Ltd. New Delhi, India, 710 pp. Online documents Cartwright J. 2007. Big stars have weather too. IOP Publishing PhysicsWeb. Available on: https://doi.org/10.1371/journal.pgen.1002204

Tables, Figures, Phothographs, Drawings

Tables and figures should be uploaded as separated files at the submission stage. Their place in the manuscript should be clearly indicated by authors. Colour figures are accepted at no charge for the electronic version. In the hardcopy version of the journal, colour figures cost (65,5 € per one colour page). When attaching files please indicate if you want colour only in the online version or in both the online and the hardcopy. Photographs and RGB bitmaps should be provided in JPG or TIFF file format. They must have no less than 300 dpi resolution. The text column should be 8 cm wide and they must be at least 1000 pixels wide. Please send original (not resized) photograph(s), straight from a digital camera, without any text descriptions on the photo. Bitmaps combined with text object descriptions should be provided in MS Word or MS Powerpoint format. Text objects using Arial font-face should be editable (changing font-face or font size). Drawings should be provided in MS Word, MS Powerpoint, CorelDRAW or EPS file format and stored with original data file. Text objects using Arial font-face should be editable (changing font-face or font size). Charts (MS Excel graphs) should be provided in MS Excel file format, and stored with original MS Excel data file without captions but with the number of the figure attached. Please do not use bitmap fills for bar charts. Use colour fills only if necessary. Captions and legends should be added at the end of the text, referred to as "Fig." and numbered consecutively throughout the paper.

Rapid communications

Rapid communications should present brief observations which do not warrant the length of a full paper. However, they must present completed studies and follow the same scientific standards as original articles. Rapid communications should contain the following sections: Title Abstract - less than 300 words Key words - maximum 6 Text body Acknowledgements References The length of such submissions is limited to 1500 words for the text, one table, and one figure.

Reviews

Review articles are invited by the editors.Unsolicited reviews are also considered. The length is limited to 5000 words with no limitations on figures and tables and a maximum of 150 references. Mini-Review articles should be dedicated to "hot" topics and limited to 3000 words and a maximum two figures, two tables and 20 references.

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