Life Sciences and Agriculture

Journal of Plant Protection Research

Content

Journal of Plant Protection Research | 2024 | vol. 64 | No 1

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Abstract

The study’s objective was to assess the efficacy and to identify the toxic mechanisms of action of some plant-derived monoterpenes and yucca extract as alternatives to chemical insecticides against the red flour beetle, Tribolium castaneum. Carvone, 1,8-cineole, cuminaldehyde, and linalool, as well as Yucca schidgera extract, were the control agents whose efficacy against the red flour beetle was tested in the laboratory and compared to malathion. The criteria for evaluating efficacy were the effects of the tested compounds on adult mortality and red flour beetle progeny. Furthermore, the effects of the control agents on some enzymes (Acetylcholinesterase, ά-amylase, and alkaline phosphatase) in T. castaneum were investigated. Moreover, the effect of the tested control measures on weight loss of treated wheat grain was studied. The tested substances showed a high ability to control T. castaneum with regard to adult mortality and offspring production, especially when used as fumigants. For adult mortality, malathion showed the highest potential against T. castaneum as a fumigant, followed by carvone, yucca extract, cuminaldehyde, linalool, and 1,8-cineole with LC 50 values of 0.05, 331.5, 365.1, 372.2, 460, and 467.5 mg · 1000 cm –2 after 72 hours, respectively. The highest reduction in progeny was for malathion followed by carvone, linalool, cineole, yucca extract, and cuminaldehyde with reduction percentages of 100, 90, 89.3,79.4 and 65.8%, respectively. The tested compounds significantly affected acetylcholinesterase activity, alpha-amylase activity, and alkaline phosphatase in the red flour beetle. There was no significant reduction in the weight of wheat grains treated with the tested materials compared to the untreated healthy grains. Finally, the tested compounds as fumigants, especially 1,8-cineole, can be considered as effective alternatives to control the red flour beetle.
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Authors and Affiliations

Aly Derbalah
1
Attiah Keratum
1
Mohamed Darwesh
1
Ahmed Fayez Omar
2
Ahmed Salama
1
Fatma Hegazy
3

  1. Pesticides Chemistry and Toxicology Department, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, Egypt
  2. Department of Stored Products Pests Research, Sakha Station, Plant Protection Research Institute, Agricultural Research Centre,Giza, Egypt
  3. Plant Protection Department, Faculty of Agriculture, Tanta University, Tanta, Egypt
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Abstract

This study aimed to evaluate the bioherbicidal effect of aqueous fresh extracts of leaves and roots of the Aloe vera plant on the broad leaf weed growth of Sonchus oleraceus associated faba bean plants. During the winters of 2020/2021 and 2021/2022, two pot experiments were carried out in the greenhouse of the National Research Center. Leaf and root aqueous extracts of Aloe vera were applied as soil and/or spray treatments at different concentrations. The results showed that there was significant inhibition in the fresh and dry biomass of S. oleraceus and was maximum with application of soil treatment (10%) of the leaf extract sequenced by spraying leaf extract at 20%. Furthermore, the inhibition of the weed growth was accompanied by an increase in the growth and yield of faba bean. The results indicated that phenols, flavonoids, alkaloids, tannins and saponins were present in the leaf extract, and there were smaller amounts of tannins and saponins in the root extract than in the leaf extract. Total phenols, flavonoids, alkaloids in the leaf extract was more than three times that of the root extract. The results also revealed that the presence of higher concentrations of natural substances in the leaf extract than in the root extracts gave it its efficiency in inhibiting the growth of S. oleraceus weeds.
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Authors and Affiliations

Kowthar Gad El-Rokiek
1
Abeer Nasr Shehata
2
Samia Ameen Saad El-Din
1
Shahira Ali Tarraf
1

  1. Botany Department, National Research Centre, Giza, Egypt
  2. Department of Biochemistry, National Research Centre, Giza, Egypt
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Abstract

This work aimed to evaluate the potential of Trichoderma asperellum organic extract and its emulsion to control cocoa black pod disease caused by Phytophthora megakarya. Organic extract was obtained after fermentation of T. asperellum and its emulsion prepared by emulsification. The in vitro antimicrobial assays of organic extract and its emulsion were evaluated and the in situ tests were carried out on detached cocoa pods. T. asperellum inhibited the mycelia growth of P. megakarya at the rates of 52% and 100%, respectively, on dual culture and the cellophane plate. This antagonist produced lytic enzymes such as cellulase, amylase, lipase and protease. The organic extract contained alkaloid, flavonoid and phenol compounds. The emulsion obtained was stable. At 100 μg · ml -1, the extract and its formulation completely inhibited the mycelial growth of P. megakarya. Similarly, when infected detached cocoa pods were sprayed with extract or emulsion, there was a significant reduction of necrosis both for healing and prevention with the latter being the most efficient. For the preventive tests, the total inhibition was recorded at 3000 μg · ml -1 and 1000 μg · ml -1, respectively, with crude organic extract and its emulsion· For curative tests, total inhibition was obtained at 4000 μg · ml -1 and 3000 μg · ml -1, respectively, for preventive and curative tests. There was a significant and positive correlation between the content of biochemical markers and the reduction of necrosis on cocoa pods after treatment with the extract or its formulation. T. asperellum organic extract emulsion could be used as an alternative in the bio- protection of cocoa black pods disease.
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Authors and Affiliations

Yves Olivier Youassi Youassi
1
Henri Tibo Ambata Ambata
1
Mylaure Benisa Jiogue
1
Fabiola Jeannette Dikongue Ndondoni
1
Moïse Ntah A Ayong
1
Marie Amperes Bedine Boat
2
Severin Tchameni Nguemezi
1
ORCID: ORCID
Modeste Lambert Sameza
1

  1. Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
  2. Department of Agriculture, Faculty of Agronomy and Agronomic Sciences, University of Dschang, Dschang, Cameroon
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Abstract

Eupatorium odoratum is known for its ability to resist nematode infestations that attack the root systems of banana plants. An aqueous extract of the leaves and stems of E. odoratum (named EEOL) represents a natural solution that we investigated for its potential to control the harmful nematode, Radopholus similis, in Cavendish banana plants. Our research into EEOL’s efficacy spanned two distinct environments: a micro plot experiment model and a field model. Various concentrations of EEOL were examined to assess its efficacy in alleviating R. similis infestations and in mitigating their adverse effects on Cavendish banana plants. In the micro plot experiment model, the concentration of the original solution, diluted at ratios of 1:30×, 1 : 16×, 1 : 8×, 1 : 4×, and 1 : 2×, ranged from 1.76 to 28.16 mg · ml -1. In the field model, the corresponding rates varied from 6.03 to 96.54 l · ha -1. Key parameters, including infection rates, root necrosis indices, plant growth metrics, percentage of fallen trees, and harvest yields, were meticulously monitored and assessed. The results demonstrated that EEOL significantly reduced infection rates ( p < 0.05), decreased root necrosis indices ( p < 0.05), and promoted increased plant height, pseudostem circumference, and leaf area ( p < 0.05) in both models. Furthermore, it lowered the percentage of fallen trees ( p < 0.05) and enhanced harvest yields ( p < 0.05) in the field model. Notably, observations in the field model revealed that EEOL, particularly at a dosage of 96.54 l · ha -1, exhibited effectiveness equivalent to the conventional chemical nematode control method, fenamiphos ( p > 0.05). The study’s findings underscore the promising potential of EEOL in effectively managing R. similis infestations and improving the yield and quality of Cavendish banana plants. The aqueous extract of the stem and leaves of E. odoratum emerged as an effective nematode management solution for banana cultivation, in both the micro plot experiment and field conditions.
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Authors and Affiliations

Thi Phuong Nhung Tran
1
Le Pham Tan Quoc
1

  1. Institute of Biotechnology and Food Technology, Industrial University of Ho Chi Minh City, Ho Chi Minh City, Viet Nam
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Abstract

This study aimed to comprehend the diversity of the fungal community on Chokanan mango, a premium mango variety from Thailand which is widely cultivated in Indonesia, associated with stem end rot and anthracnose disease using high-throughput amplicon targeted metagenomics analysis by next-generation sequencing (NGS). Samples used in this study were freshly harvested healthy fruits at the age of 15-weeks (H15.ITS), healthy fruits after 2 weeks incubation (H17.ITS), 17-week old fruits (S17.ITS) with stem end rot symptoms, and 17-week old fruits (A17.ITS) with anthracnose symptoms. Results showed that the Basidiomycota phylum was dominant in the healthy fruits, while the Ascomycota phylum was found dominantly in sick fruits. Based on OTUs alignment of sequenced data, some species found to be dominantly associated with stem end rot disease in this study were Lasiodiplodia theobromae, Neofusicoccum cordaticola and N. mangiferae. Dominant species which were associated with mango anthracnose disease were Colletotrichum gloeosporioides, Botryosphaeria corticis, Volutella sp., and Pseudofusicoccum violacearum. These fungal genera were not found to be dominant in healthy fruits at the same age indicating that specific genera contributed to developing postharvest diseases on mango differently. The findings confirmed that the fungal community associated with stem end rot and anthracnose disease on mango was unique, and specific species contributed in particular disease development. Since mango is an important global commodity, these research findings will contribute significantly to global biosecurity.
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Authors and Affiliations

Ani Widiastuti
1
ORCID: ORCID
Suryanti
1
Alvina Clara Giovanni
1
Niken Rasmi Paramita
2

  1. Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
  2. Galasari Gunung Sejahtera, Gresik, East Java, Indonesia
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Abstract

In the years 2018‒2020, the effectiveness of three synthetic active substances (acequinocyl, fenpyroximate, spirodiclofen), one substance derived from Streptomyces spp. (abamectin), a plant extract (orange oil) and silicone polymers in controlling Phyllocoptes gracilis in two Polish raspberry plantations (v. ‘Glen Ample’) was assessed. All the substances showed high and comparable efficacy against the tested pest, significantly reducing its population. However, their effects occurred at different times after the application. The strongest immediate control was shown by silicone polymers, followed by abamectin and spirodiclofen. The full effect of fenpyroximate application was visible after approx. 2 weeks, while acequinocyl was effective 3‒4 weeks after the application. Moreover, the content of phenolic compounds, sterols and triterpenoids was determined in leaves of plants treated with spirodiclofen, orange oil and silicone polymers. The observed increase in the content of salicylic acid and changes in the content of triterpenoids in leaves may indicate a stimulating effect of the substances to the natural defense processes of plants.
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Authors and Affiliations

Gerard Podedworny
1
Małgorzata Tartanus
1
Danuta Solecka
2
Anna Szakiel
3
Eligio Malusà
1

  1. Department of Plant Protection, National Institute of Horticultural Research in Skierniewice, Skierniewice, Poland
  2. Institute of Experimental Plant Biology and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland
  3. Institute of Biochemistry, Faculty of Biology, University of Warsaw, Warsaw, Polandd
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Abstract

Seed coating technology combined with biopolymers offers an alternative method to reduce environmental contamination. However, when biological agents are incorporated, biopolymers would have diverse properties and effects. This underscores the necessity of exploring the optimal dosages and formulations of biopolymers to ensure the survival of beneficial microorganisms, seed quality, and proper storage. This study aimed to explore the effects of different sodium alginate and chitosan coating formulations on Trichoderma harzianum viability and canola seeds quality. The coating process involved mixing T. harzianum powder with sodium alginate, talc and chitosan in different doses, sequences and formulations. Trichoderma harzianum viability was assessed through colony-forming units per ml over time. Canola seed quality was evaluated by measuring radicle emergence, germination percentage, seedling growth, and field emergence. Sodium alginate, both alone and in combination with talc, improved T. harzianum viability immediately after treatment and during storage. These coatings did not impair seed germination and improved canola root growth. Among the different chitosan formulations, a 1 : 100 ratio in talc improved strain survival and root growth without affecting germination, radicle, and field emergence. Coating canola seeds is a practical alternative to the application of T. harzianum, sodium alginate and talc, as it preserves their viability over time and improves seedling performance. Chitosan formulations in acetic acid should be carefully developed to prevent negative effects on seeds or biological agents.
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Authors and Affiliations

Cyntia Lorena Szemruch
1
ORCID: ORCID
Marta Monica Astiz Gassó
2
Federico Augusto García
1
Carola Gonçalves Vila Cova
3
Silvia Sanchez
3
Yanina Ibáñez
4
Antonella Colinas
3

  1. Faculty of Agricultural Sciences, Institute of Research on Agricultural Production, Environment, and Health (IIPAAS), National University of Lomas de Zamora, Llavallol, Argentina
  2. Phytotechnical Institute of Santa Catalina, National University of La Plata, La Plata, Argentina
  3. Faculty of Agricultural Sciences, National University of Lomas de Zamora, Llavallol, Argentina
  4. Faculty of Agricultural Sciences, Institute of Research on Agricultural Production,Environment, and Health (IIPAAS), National University of Lomas de Zamora, Llavallol, Argentina
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Abstract

The occurrence of three bamboo aphid species of the genus Takecallis was detected. T. arundicolens (Clarke) and T. arundinariae (Essig) were recorded for the first time in Poland, and new localities for T. nigroantennatus Wieczorek were found. Key diagnostic morphological characteristics to help distinguish these species and DNA barcoding to analyze individuals at the molecular level were provided.
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Authors and Affiliations

Karina Wieczorek
1
Kaja Ball
1
Roma Durak
2
Beata Borowiak-Sobkowiak
3

  1. Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Katowice, Poland
  2. Institute of Biology, University of Rzeszów, Rzeszów, Poland
  3. Department of Entomology and Environmental Protection, Poznań University of Life Sciences, Poznań, Poland
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Abstract

For the first time, the mycophagous beetle Sericoderus lateralis (Gyllenhal, 1827) was documented feeding on tomato leaf mold ( Cladosporium fulvum Cooke, 1878) tissues. The phenomenon was observed during the years 2022 and 2023 in a hydroponic tomato greenhouse situated near the Czech-Polish border within the cadastre of Dolní Lutyně municipality in Czechia. Greenhouse and laboratory observations confirmed that adult and larvae feeding activity led to a reduction in tomato leaf mold lesions. In addition, there was a positive correlation between tomato leaf mold disease progression and increased populations of S. lateralis in the tomato crop. Petri dish observations confirmed egg laying occurred on a diet of tomato leaf mold. Further research is warranted to discern whether S. lateralis is a potential biological control agent for tomato leaf mold or if it acts to facilitate the spread of the disease by acting as a spore vector.
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Authors and Affiliations

Václav Psota
1
ORCID: ORCID
Jan Bezděk
2
ORCID: ORCID
Liam Harvey
3

  1. Production Greenhouse, Farma Bezdínek s.r.o., Czech Republic
  2. Department of Zoology, Fisheries, Hydrobiology and Apiculture, Mendel University in Brno, Brno, Czech Republic
  3. IPS Department, Biobest Group NV, Belgium
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Abstract

Fruit flies belonging to the Bactrocera dorsalis species complex pose a significant threat to mangoes and other crops in the Philippines and worldwide. Identifying cryptic species within this complex is challenging, particularly when relying solely on morphological analysis. In this study, we sequenced two fragments of the nuclear 18S and ITS2-28S rRNA genes from specimens of Bactrocera dorsalis Hendel collected in the Philippines to assess their applicability for species diagnostics. Subsequent sequencing and analysis revealed that the 18S and 28S rRNA gene fragments matched B. dorsalis sequences in NCBI but also displayed high similarity with other Bactrocera and insect species. On the other hand, sequences of the ITS2 segment showed hits specific to B. dorsalis. Further analysis of the 18S rRNA gene in fruit flies collected from various sources and host plants in the country suggests conserved sequences among Bactrocera samples, irrespective of collection site and host plant species. In conclusion, our findings suggest that, among the tested nuclear DNA fragments, only the ITS2 demonstrates sufficient species-level nucleotide variation for effective use as a molecular diagnostic marker for B. dorsalis identification.
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Authors and Affiliations

Cris Q. Cortaga
1
ORCID: ORCID
Maria Luz J. Sison
1

  1. Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños, 4031 College, Laguna, Philippines

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Instructions for Authors

Manuscripts published in JPPR are free of charge. Only colour figures and photos are payed 61.5 € per one colour page JPPR publishes original research papers, short communications, critical reviews, and book reviews covering all areas of modern plant protection. Subjects include phytopathological virology, bacteriology, mycology and applied nematology and entomology as well as topics on protecting crop plants and stocks of crop products against diseases, viruses, weeds, etc. Submitted manuscripts should provide new facts or confirmatory data. All manuscripts should be written in high-quality English. Non-English native authors should seek appropriate help from English-writing professionals before submission. The manuscript should be submitted only via the JPPR Editorial System (http://www.editorialsystem.com/jppr). The authors must also remember to upload a scan of a completed License to Publish (point 4 and a handwritten signature are of particular importance). ALP form is available at the Editorial System. The day the manuscript reaches the editors for the first time is given upon publication as the date ‘received’ and the day the version, corrected by the authors is accepted by the reviewers, is given as the date ‘revised’. All papers are available free of charge at the Journal’s webpage (www.plantprotection.pl). However, colour figures and photos cost 61.5 € per one colour page.

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