Nauki Biologiczne i Rolnicze

Journal of Plant Protection Research

Zawartość

Journal of Plant Protection Research | 2024 | vol. 64 | No 3

Abstrakt

Sheath blight disease in rice, induced by the necrotrophic basidiomycetes Rhizoctonia solani, has emerged as a significant menace to global rice cultivation, especially with the widespread adoption of high-yielding varieties. The pathogen’s capacity to endure unfavorable conditions and its wide host range contribute to the increased challenge for management. The occurrence of sheath blight in rice is intensified when high-yielding semi-dwarf cultivars are employed, coupled with dense planting and the application of substantial amounts of nitrogenous fertilizers. Managing this pathogen is a formidable challenge due to its broad host range, substantial genetic variability, and the absence of satisfactory levels of natural resistance in the existing rice germplasm. Due to the absence of complete resistance sources, the predominant approach for managing sheath blight has been through chemical control methods. It is imperative to explore solutions to combat this pathogen, in order to reduce rice yield losses and safeguard global food security. Developing genetic resistance provides an alternative to the use of potentially harmful chemical fungicides. This review crucially delivers efforts to enhance the understanding of the host–pathogen relationship, which involves identification of gene loci/markers associated with resistance responses, modification of host genome through transgenic approaches, examining the wide host range, epidemiology and its managemental approaches. Recent advancements and current research on the R. solani–rice pathosystem, along with a gap analysis, are presented.
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Autorzy i Afiliacje

Kumar Avinash Biswal
1
Siddhartha Das
1
ORCID: ORCID

  1. Department of Plant Pathology, MS Swaminathan School of Agriculture, Centurion University of Technology and Management,Paralakhemundi, India

Abstrakt

Diaphorina citri, an important pest and insect vector that can transmit the pathogenic bacteria Candidatus Liberibacter asiaticus, causing Huanglongbing disease, is one of many challenges in citrus agriculture. Integrated pest management by utilizing microorganisms is a wise and efficient alternative without damaging the environment. Utilization of Plant Growth Promoting Rhizobacteria (PGPR), such as Bacillus cereus and B. velezensis, is a potential strategy for the biological control of plant diseases or insect vectors. By inducing systemic resistance in plants, PGPR can enhance plant defense against diseases and insect pests while activating molecular and physiological changes in plants. This research aimed to determine the effect of B. cereus and B. velezensis on the plant growth and feeding behavior of D. citri. The height and volume of the plant canopy were observed periodically for 6 months, while the feeding behavior of D. citri was monitored using the Electrical Penetration Graph (DC-EPG). The results showed increased height and volume of the citrus plant canopy treated with B. cereus, indicating that B. cereus could act as a PGPR. The application of B. cereus and B. velezensis to citrus seedlings affected the feeding behavior of D. citri. D citri showed difficulty in penetrating the phloem tissue of citrus plants.
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Autorzy i Afiliacje

Ike Marisna
1
Alan Soffan
1
ORCID: ORCID
Siti Subandiyah
1
Yijing Cen
2
Tri Joko
1
ORCID: ORCID

  1. Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
  2. Laboratory of Insect Ecology, South China Agricultural University, Guangzhou, China

Abstrakt

The cotton aphid Aphis gossypii is a major agricultural pest of cotton that causes substantial damage to the crop not only by sucking sap but also through virus transmission. Globally adopted traditional and contemporary approaches to control aphid infestation have certain limitations and are hazardous to human health. RNA interference (RNAi) technology has unfolded its potential as an effective crop protection strategy against various pests. In this study, we adopted plant-mediated RNAi strategy to enhance aphid resistance in cotton by targeting arginine kinase (AK), which is a crucial enzyme responsible for energy homeostasis in insects. We selected a 312bp dsRNA fragment containing eight siRNAs and showing optimum GC content, Hb index, and stable secondary structure based on computational prediction studies. The binary construct expressing dsRNA was used to transform local cotton variety MNH886 and four transgenic lines were obtained in the T1 generation. Out of the four T1 transgenic cotton lines, dsA-7 exhibited the highest aphid mortality (73.3%), whereas, dsA-1, dsA-3 and dsA-6 revealed 60%, 61%, and 66.6% aphid mortality, respectively, in comparison to 13.3% mortality in the mock control cotton line. Moreover, significant knockdown in mRNA expression of AK was observed in aphids fed dsA-7 which was 79%. In comparison, 54%, 47%, and 45% downregulation was recorded in aphids which fed on dsA-6, dsA-3, and dsA-1 transgenic cotton lines, respectively. These results revealed that plant-mediated downregulation of aphid RNA induced significant RNA interference in A. gossypii which resulted in considerable aphid mortality and led to plant protection against aphids.
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Autorzy i Afiliacje

Iqra Yousaf
1
Bushra Tabassum
2
ORCID: ORCID
Basit Jabbar
1
Muhammad Arslan Amjad
3
Uzma Qaisar
2
Anwar Khan
4
ORCID: ORCID
Rida Khalid
2
Olawale Samuel Adeyinka
5
Idrees Ahmad Nasir
1

  1. Centre of Excellence in Molecular Biology, University of the Punjab Lahore, Lahore, Pakistan
  2. School of Biological Sciences, University of the Punjab Lahore Pakistan, Quaid e Azam campus, Lahore, Pakistan
  3. Institute of Molecular Biology & Biotechnology, The University of Lahore, Raiwind Road, Lahore, Pakistan
  4. Department of Microbiology, The Balochistan University of Information Technology, Engineering, and Management Sciences, Quetta, Pakistan
  5. Department of Chemistry, University of Alabama Huntsville, Hunsville, United States

Abstrakt

The glycosyl hydrolase β-glucanase elicits plant immune responses against pathogens and enhances plant immunity by activating signaling pathways. The specific functional domains responsible for disease prevention remain unclear. In this study, transient expression of β-glucanase significantly increased leaves resistance to Botrytis cinerea in Nicotiana benthamiana systemic leaves. Through sequence alignment and similarity analysis, five conserved motifs in the amino acid sequence of β-glucanase were identified, and five deletion mutants were generated to investigate its essential regions further. Notably, the N-terminal amino acid sites 5-54 deletion mutation of β-glucanase decreased resistance to B. cinerea infection. These results indicate that N-terminal amino acids 5-54 (N54) are crucial for β-glucanase induced N. benthamiana defense response and for enhancing resistance to B. cinerea. Further analysis using real-time quantitative fluorescent PCR (qRT-PCR) revealed a significant reduction in gene expression within the N54 region compared to that of unmutated β-glucanase. Additionally, there was a notable reduction in the relative expression levels of FRK, CYP71D20, WRKY7, WRKY8, ACRE31 and PTI genes. Therefore, the first 50 amino acids at positions 5-54 within the N-terminal domain were essential for triggering plant defense responses and enhancing resistance against B. cinerea infection. This study provides an important theoretical foundation for systematic investigation into key functional domains within β-glucanase that trigger defensive responses in plants against B. cinerea.
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Autorzy i Afiliacje

Qi Sun
1
Tong Sun
1
Yujin Yuan
1
Ruokui Chang
2
Weiwei Yu
1
Xiaohui Qiu
1
Tianyi Wu
1
Anling Deng
1
Zhuoran Li
1
Yuanhong Wang
1

  1. Horticulture and Landscape Architecture, Tianjin Agriculture University, Tianjin, China
  2. College of Engineering and Technology Architecture, Tianjin Agriculture University, Tianjin, China

Abstrakt

The cocoa tree is prone to diverse diseases such as stem borer, stem canker, swollen shot, and root rot disease which impedes high yield. Early disease detection is a critical component of diverse management processes that are implemented throughout the life cycle of cocoa plants. Consequently, several studies on the application of detection techniques to recognize diseases have been proposed by several researchers. This study proposes the YOLOv5m network for cocoa tree disease detection. The development of cocoa disease detection systems will aid farmers in early identification prompt response, and efficient management of related cocoa tree diseases which will ultimately increase yield and sustainability. To improve the performance of the YOLOv5m network, a Swin Transformer (Swin-T) was added to the backbone network to improve cocoa tree disease detection accuracy. By obtaining global information, the K-means++ algorithm was added to modify the choice of initial clustering locations, and Efficient Intersection over Union Loss (EIoU) was used as a bounding box regression loss function to speed up the bounding box regression rate, resulting in higher precision of the YOLOv5m network. The experimental assessment outcome of this study showed that the proposed method YOLOv5m (Swin-T, K-means++, EIoU) achieved 96% precision, mAP of 92%, and recall of 94%. Compared to the original YOLOv5m, precision improved by 5%, mAP improved by 6%, and recall by 5%. Comparing the proposed method to the conventional YOLOv5m, the latter showed improved performance and better accuracy with a high detection speed and compactness. This improvement offers a useful and effective method for detecting diseases related to cocoa trees.
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Autorzy i Afiliacje

Benedicta Nana Esi Nyarko
1
ORCID: ORCID
Wu Bin Wu
1
Zhou Jinzhi Zhou
1
Mwanaharusi Mohd Juma Mohd
1

  1. School of Information Engineering, Southwest University of Science and Technology, Qinglong Road, Fucheng District, Mianyang C, Mianyang, China

Abstrakt

The lesser grain borer (Rhyzopertha dominica F.) and the rice weevil (Sitophilus oryzae L.) are stored grain pests that cause significant economic losses in grain storage. This study aimed to analyze the impact of the chemical composition of wheat and barley grain (e.g., protein, fatty acids and total antioxidant capacity) on the development of two species of storage pests and to determine the relationship between the analyzed variables. The study involved the evaluation of 10 wheat cultivars and 10 barley cultivars under laboratory conditions. The observations included assessing the beetles’ progeny abundance, dust mass produced after feeding, and grain mass loss. The chemical composition of the tested wheat and barley cultivars was also determined, and the influence of different chemical compositions on insect development was investigated. The results of the experiment revealed diversity of resistance among cultivars to pest feeding. Larger populations of the lesser grain borer were observed on barley grains, while rice weevil populations were higher on wheat. Cultivars with higher protein and fat content were more susceptible to pest attacks. A connection between the amount of dust, grain mass loss, and the type of pest was also identified, indicating differences in feeding mechanisms and selective food preferences of these insects. The grain chemistry of wheat cultivars, including the content of fatty acids and antioxidants, significantly influenced the progeny abundance of S. oryzae, suggesting the potential of these components as natural barriers against storage pests. This study provides valuable insights for developing breeding strategies to enhance the natural resistance of new grain cultivars to these pests, contributing to the reduction of pesticide use. Statistical analyses confirmed the significance of differences in grain composition in varied resistance to the studied pests. The conclusions drawn from this work may help establish new storage and breeding practices, promoting sustainable agriculture and protecting natural resources.
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Autorzy i Afiliacje

Olga Kosewska
1
ORCID: ORCID
Mariusz Nietupski
1
ORCID: ORCID
Stanisława Koronkiewicz
1
ORCID: ORCID
Sebastian Wojciech Przemieniecki
1
ORCID: ORCID

  1. Faculty of Agriculture and Forestry, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

Abstrakt

Citronella (Cymbopogon nardus) produces essential oil and has the potential to be developed as a botanical insecticide. However, in its development, botanical insecticides encountered several obstacles. Utilizing nanotechnology in nanoemulsion preparations is one method to overcome these challenges. This research aimed to determine the contents of the citronella oil nanoemulsion (CiONano) and citronella oil non-nano emulsion (CiONonNano) formulations and evaluate the toxicity, repellency, and prevention of oviposition against female adults of Callosobruchus maculatus. This was the first work to evaluate the nanoemulsion of citronella oil prepared from spontaneous emulsification against C. maculatus. Chemical content testing used the GCMS method. A toxicity test using the contact method (LC50), used a probit program, while testing for repellency and oviposition deterrence was carried out using the no-choice method. The highest chemical component of CiONonNano and CiONano was citronella (37.56 and 38.97%, respectively), followed by citronellol (17.71 and 18.99%, consecutively) and geraniol (14.78 and 15.38%, respectively). In general, the CiONano formulation showed higher repellency and toxicity than CiONonNano. The LC50 values of CiONano were 10.03%. These values were 4.49 times lower than the LC50 of CiONonNano. However, the results of the oviposition inhibition test showed different results, the CiONonNano formulation had a higher oviposition inhibition capacity for adult female C. maculatus than CiONano. As a result, it is necessary to optimize the CiONano formulation to obtain consistent results in controlling C. maculatus.
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Autorzy i Afiliacje

Rohimatun
1
ORCID: ORCID
Mahindra Dewi Nur Aisyah
2
ORCID: ORCID
Rismayani
1
ORCID: ORCID
Molide Rizal
1
ORCID: ORCID
Rita Noveriza
1
ORCID: ORCID
Tri Lestari Mardiningsih
1
ORCID: ORCID

  1. Research Center for Estate Crops, Organization Research for Agriculture and Food, National Research and Innovation Agency (BRIN), Cibinong, Indonesia
  2. Agricultural Production, Politeknik Negeri Jember, Jember, Indonesia

Abstrakt

The aim of this study conducted in 2018–2020 was to determine the effect of biostimulants and herbicide Avatar 293 ZC on the occurrence of external and internal defects and small tubers in the potato yield. The edible cultivars evaluated were Oberon and Malaga and the biostimulants used were: PlonoStart containing prolactic acid bacteria, actinomycetes (N-16.4%, K2O-0.75%, CaO-0.07%, MgO-0.02%, S-941 mg · kg−1), Aminoplant containing free amino acids-11.57%, organic matter-87.7% (Ntotal-9.48%, Norganic-9.2%, N-NH4- 0.88%, Corganic-25.0%), and Agro-Sorb Folium including total amino acids-13.11%, free amino acids-10.66% (N-2.2%, B-0.02%, Mn-0.05%, Zn-0.09%) with herbicides (clomazone and metribuzin): objects 3,4 and 5 and a single herbicide (object 2), as well as a control object (1). Before harvesting, tubers were collected from 10 randomly selected plants from each plot. The yield structure was determined in these samples - the weight share of tubers below 35 mm, 36–50, 51–60 and above 60 mm. Tubers with a diameter of less than 35 mm were classified as small, non-commercial tubers. Tubers with a diameter above 35 mm constituted the commercial fraction, in which external and internal defects were determined. The sum of tubers with defects and small tubers constituted side yield. The use of all biostimulants with herbicide significantly reduced: the share of tubers with defects, the share of small tubers in the yield and the total weight of small tubers and tubers with defects compared to the control plant. The best effects in reducing the occurrence of tubers with external and internal defects and small tubers were obtained by using the Agro-Sorb Folium and herbicide. Among the cultivars, Malaga cv. turned out to be more resistant to defects and the production of small tubers than the Oberon cv. In the literature, especially foreign, there are sporadic studies on the effect of biostimulants on the occurrence of defects in potato tubers.
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Autorzy i Afiliacje

Agnieszka Ginter
1
ORCID: ORCID
Krystyna Zarzecka
1
ORCID: ORCID
Marek Gugała
1
ORCID: ORCID
Iwona Mystkowska
2
ORCID: ORCID

  1. Faculty of Agricultural Sciences, University of Siedlce, Siedlce, Poland
  2. Department of Dieteties, Bialska Academy of John Paul II, Biała Podlaska, Poland

Abstrakt

Sustainable crop cultivation is a crucial goal in modern agriculture, aiming to attain high productivity while conserving natural resources. This requires the implementation of rational cultivation techniques, with proper irrigation and fertilization practices playing a crucial role in ensuring plant well-being and providing natural protection against biotic and abiotic stresses. The health-promoting properties of crops are also significantly influenced by irrigation and fertilization. This study investigated the productivity, nutritional composition, bioactive compounds, and antioxidant capacity of Vineta early potato cultivar tubers under drip irrigation conditions, combined with nitrogen fertilization through fertigation or broadcasting. Two-factor trials included drip irrigation (control or drip) and nitrogen application (broadcast or fertigation). Precise treatments, such as drip irrigation and N-fertigation were found to enhance all productivity traits. Both practices positively impacted tuber nutrient content. The highest levels of total polyphenols and chlorogenic acid were observed in non-irrigated and broadcasted tubers. Drip-irrigated and N-fertigated tubers exhibited high levels of vitamin C and antioxidant activity. In summary, the combination of drip irrigation with appropriate fertilization methods positively influenced potato crops, as evidenced by an increase in productivity and the quality of tubers.
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Autorzy i Afiliacje

Tomasz Jakubowski
1
ORCID: ORCID
Stanisław Rolbiecki
2
ORCID: ORCID
Roman Rolbiecki
2
ORCID: ORCID
Dorota Wichrowska
3
Anna Figas
4
Barbara Jagosz
5
ORCID: ORCID
Atilgan Atilgan
6
Ferenc Pal-Fam
7
ORCID: ORCID
Sandor Keszthelyi
7
Anna Krakowiak-Bal
8

  1. Department of Machine Operation, Ergonomics and Production Processes, University of Agriculture in Krakow, Krakow, Poland
  2. Department of Agrometeorology, Plant Irrigation and Horticulture, Bydgoszcz University of Science and Technology, Bydgoszcz, Poland
  3. Department of Microbiology and Food Technology, Faculty of Agriculture and Biotechnology, Bydgoszcz University of Science and Technology, Bydgoszcz, Poland
  4. Department of Biotechnology, Faculty of Agriculture and Biotechnology, Bydgoszcz University of Science and Technology, Bydgoszcz, Poland
  5. Department of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Kraków, Poland
  6. Department of Biosystems Engineering, Alanya Alaaddin Keykubat University, Üniversite Caddesi, Alanya, Turkey
  7. Institute of Plant Production, University of Agriculture and Life Sciences, Páter Károly, Gödöllő, Hungary
  8. Department of Bioprocess, Power Engineering and Automation, University of Agriculture in Krakow, Kraków, Poland

Abstrakt

Plant-parasitic nematodes of the genus Xiphinema Cobb, 1913 constitute a group of soilinhabiting, polyphagous ectoparasites of plant roots, some of which are also vectors of nepoviruses. In this study, 1237 soil samples were taken from different regions of Poland. In two of these samples (0.16% of all collected) Xiphinema parataylori Lazarova et al., 2019 was present, which constitutes not only the first record from Poland but also the first report outside the Czech Republic and Slovakia, where this species was initially described. Specimens from Poland were largely similar to those from the original description except for a slenderer body (maximal body thickness in two populations found 44.8 and 46.8 vs 54, 55 and 56 μm resulting in higher ‘a’ index: 50.0 and 50.8 vs 37.8, 40.7 and 41.2). Specimens from the Polish populations were also characterized based on the ITS1 molecular marker. Finally, both populations recorded from Poland were associated with wild pear (Pyrus pyraster) extending the number of known host plants.
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Autorzy i Afiliacje

Franciszek Kornobis
1
ORCID: ORCID
Arnika Przybylska
2
ORCID: ORCID

  1. Department of Entomology and Animal Pests, Institute of Plant Protection – National Research Institute, Poznań, Poland
  2. Department of Molecular Biology and Biotechnology, Institute of Plant Protection – National Research Institute, Poznań, Poland

Abstrakt

The aim of this study was to investigate insect derived bacteria for the ability to dissolve insoluble soil phosphate to release soluble phosphorus compounds, available to plants. Bacterial isolates were obtained from Diabrotica virgifera, Hermetia illucens, Oulema melanopus, and Ostrinia nubilalis. An in vitro evaluation of phosphate solubilization ability on Pikovskaya’s medium was done and the phosphate solubilizing index (PSI) was calculated for each isolate. Bacteria were tested in a greenhouse experiment on seeds of oats, wheat, triticale, barley and soybeans. After incubation, the weight and length of their aerial plant parts were measured. The highest increase in the weight of aerial parts was recorded for oats after using strain Om046 for inoculation (88.98%), then, wheat (Dv097, 31.43%), soybean (strain 96, 53.79%), and triticale (bacterial consortium, 36.9%). Bacteria used were identified as Lactococcus lactis (strains Om030 and Om046), Acinetobacter sp. (Dv123), Lactococcus garvieae (Dv097) and Rothia kristinae (strains 90 and 96). We showed that a successful application of insect derived bacteria for phosphate solubilization in soil, to promote plant growth, is possible. Innovative agriculture requires constant improvements in increasing crop growth. Thus, new sources of bacterial strains effectively promoting plant growth, are needed. We described a new source of plant growth-promoting bacteria that can be used in agriculture.
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Autorzy i Afiliacje

Weronika Zenelt
1
Agata Pruciak-Nowak
2
ORCID: ORCID
Krzysztof Krawczyk
3
ORCID: ORCID

  1. Plant Disease Clinic and Bank of Plant Pathogen, Institute of Plant Protection – National Research Institute, Poznań, Poland
  2. Research Centre of Quarantine, Invasive and Genetically Modified Organisms, Institute of Plant Protection – National Research Institute, Poznań, Poland
  3. Virology and Bacteriology Department, Institute of Plant Protection – National Research Institute, Poznań, Poland

Instrukcja dla autorów

Instructions for Authors

Manuscripts published in JPPR are free of charge. Only colour figures and photos are payed 61.5 € per one colour page JPPR publishes original research papers, short communications, critical reviews, and book reviews covering all areas of modern plant protection. Subjects include phytopathological virology, bacteriology, mycology and applied nematology and entomology as well as topics on protecting crop plants and stocks of crop products against diseases, viruses, weeds, etc. Submitted manuscripts should provide new facts or confirmatory data. All manuscripts should be written in high-quality English. Non-English native authors should seek appropriate help from English-writing professionals before submission. The manuscript should be submitted only via the JPPR Editorial System (http://www.editorialsystem.com/jppr). The authors must also remember to upload a scan of a completed License to Publish (point 4 and a handwritten signature are of particular importance). ALP form is available at the Editorial System. The day the manuscript reaches the editors for the first time is given upon publication as the date ‘received’ and the day the version, corrected by the authors is accepted by the reviewers, is given as the date ‘revised’. All papers are available free of charge at the Journal’s webpage (www.plantprotection.pl). However, colour figures and photos cost 61.5 € per one colour page.

General information for preparing a manuscript

All text should be written in a concise and integrated way, by focusing on major points, findings, breakthrough or discoveries, and their broad significance. All running text should be in Times New Roman 12, 1.5 spacing with all margins 2.5 cm on all sides.

Original article

The original research articles should contain the following sections: Title – the title should be unambiguous, understandable to specialists in other fields, and must reflect the contents of the paper. No abbreviations may be used in the title. Name(s) of author(s) with affiliations footnoted added only to the system, not visible in the manuscript (Double Blind Reviews). The names of the authors should be given in the following order: first name, second name initial, surname. Affiliations should contain: name of institution, faculty, department, street, city with zip code, and country. Abstract – information given in the title does not need to be repeated in the abstract. The abstract should be no longer than 300 words. It must contain the aim of the study, methods, results and conclusions. If used, abbreviations should be limited and must be explained when first used. Keywords – a maximum of 6, should cover the most specific terms found in the paper. They should describe the subject and results and must differ from words used in the title. Introduction – a brief review of relevant research (with references to the most important and recent publications) should lead to the clear formulation of the working hypothesis and aim of the study. It is recommended to indicate what is novel and important in the study. Materials and Methods – in this section the description of experimental procedures should be sufficient to allow replication. Organisms must be identified by scientific name, including authors. The International System of Units (SI) and their abbreviations should be used. Methods of statistical processing, including the software used, should also be listed in this section. Results – should be presented clearly and concisely without deducting and theori sing. Graphs should be preferred over tables to express quantitative data. Discussion – should contain an interpretation of the results ( without unnecessary repetition) and explain the influence of experimental factors or methods. It should describe how the results and their interpretation relate to the scientific hypothesis and/or aim of the study. The discussion should take into account the current state of knowledge and up-to-date literature. It should highlight the significance and novelty of the paper. It may also point to the next steps that will lead to a better understanding of the matters in question. Acknowledgements – of people, grants, funds, etc. should be placed in a separate section before the reference list. The names of funding organizations should be written in full. References In the text, papers with more than two authors should be cited by the last name of the first author, followed by et al. (et al. in italics), a space, and the year of publication (example: Smith et al. 2012). If the cited manuscript has two authors, the citation should include both last names, a space, and the publication year (example: Marconi and Johnston 2006). In the Reference section, a maximum of ten authors of the cited paper may be given. All references cited in the text must be listed in the Reference section alphabetically by the last names of the author(s) and then chronologically. The year of publication follows the authors’ names. All titles of the cited articles should be given in English. Please limit the citation of papers published in languages other than English. If necessary translate the title into English and provide information concerning the original language in brackets (e.g. in Spanish). The list of references should only include works from the last ten years that have had the greatest impact on the subject. Older references can be cited only if they are important for manuscript content. The full name of periodicals should be given. If possible, the DOI number should be added at the end of each reference. The following system for arranging references should be used: Journal articles Jorjani M., Heydari A., Zamanizadeh H.R., Rezaee S., Naraghi L., Zamzami P. 2012. Controlling sugar beet mortality disease by application of new bioformulations. Journal of Plant Protection Research 52 (3): 303-307. DOI: https://doi.org/10.2478/v10045-012-0049-9 Online articles Turner E., Jacobson D.J., Taylor J.W. 2011. Genetic architecture of a reinforced, postmating, reproductive isolation barrier between Neurospora species indicates evolution via natural selection. PLoS Genetics 7 (8): e1002204. DOI: https://doi.org/10.1371/journal.pgen.1002204 Books Bancrof J.D., Stevens A. 1996. Theory and Practice of Histological Techniques. 4th ed. Churchill Livingstone, Edinburgh, UK, 776 pp. Book chapters Pradhan S.K. 2000. Integrated pest management. p. 463-469. In: "IPM System in Agriculture. Cash Crop" (R.K. Upadhyaya, K.G. Mukerji, O.P. Dubey, eds.). Aditya Books Pvt. Ltd. New Delhi, India, 710 pp. Online documents Cartwright J. 2007. Big stars have weather too. IOP Publishing PhysicsWeb. Available on: https://doi.org/10.1371/journal.pgen.1002204

Tables, Figures, Phothographs, Drawings

Tables and figures should be uploaded as separated files at the submission stage. Their place in the manuscript should be clearly indicated by authors. Colour figures are accepted at no charge for the electronic version. In the hardcopy version of the journal, colour figures cost (65,5 € per one colour page). When attaching files please indicate if you want colour only in the online version or in both the online and the hardcopy. Photographs and RGB bitmaps should be provided in JPG or TIFF file format. They must have no less than 300 dpi resolution. The text column should be 8 cm wide and they must be at least 1000 pixels wide. Please send original (not resized) photograph(s), straight from a digital camera, without any text descriptions on the photo. Bitmaps combined with text object descriptions should be provided in MS Word or MS Powerpoint format. Text objects using Arial font-face should be editable (changing font-face or font size). Drawings should be provided in MS Word, MS Powerpoint, CorelDRAW or EPS file format and stored with original data file. Text objects using Arial font-face should be editable (changing font-face or font size). Charts (MS Excel graphs) should be provided in MS Excel file format, and stored with original MS Excel data file without captions but with the number of the figure attached. Please do not use bitmap fills for bar charts. Use colour fills only if necessary. Captions and legends should be added at the end of the text, referred to as "Fig." and numbered consecutively throughout the paper.

Rapid communications

Rapid communications should present brief observations which do not warrant the length of a full paper. However, they must present completed studies and follow the same scientific standards as original articles. Rapid communications should contain the following sections: Title Abstract - less than 300 words Key words - maximum 6 Text body Acknowledgements References The length of such submissions is limited to 1500 words for the text, one table, and one figure.

Reviews

Review articles are invited by the editors.Unsolicited reviews are also considered. The length is limited to 5000 words with no limitations on figures and tables and a maximum of 150 references. Mini-Review articles should be dedicated to "hot" topics and limited to 3000 words and a maximum two figures, two tables and 20 references.

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