Life Sciences and Agriculture

Journal of Plant Protection Research

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Abstract

The Solanaceae family includes many species of plants with high nutritional and medicinal value. Plants in this family have evolved towards diversifying specialized (secondary) metabolism to adapt to adverse conditions, and a few of them have been used as model plants in the study of plant defense. 2-Methylketones are insecticidal compounds that accumulate in certain plants, particularly in wild tomato Solanum habrochaites f. glabratum – a Solanaceae member. 2-Methylketones are the decarboxylated products of 3-ketoacids generated through the hydrolysis of 3-ketoacyl-ACPs by methylketone synthase 2 (MKS2). In this study, we isolated one of the four MKS2 homolog genes from the cultivated potato Solanum tuberosum and designated it as StMKS2-3. A combined in silico approach including sequence alignment, phylogenetic assessment, 3D structural modeling and RNA-sequencing data analysis was performed to exploit the functional properties of this gene. The encoded protein possesses the conserved Aspartate and functional domain characteristic of single Hotdog-fold thioesterases, and it shares high similarity in sequence and structure with SlMKS2a of the cultivated tomato (S. lycopersicum). This suggests that, like SlMKS2a, StMKS2-3 could act as a 3-ketoacyl-ACP thioesterase. According to RNA-seq data, StMKS2-3 exhibited higher expression than the other three StMKS2 genes in most tissues across different potato S. tuberosum cultivars. The quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that StMKS2-3 was expressed in multiple potato tissues, both aerial and root parts, but the highest expression was in tubers and sprouts. Furthermore, this gene appeared to be transcriptionally induced in response to salinity, drought, and Phytophthora infestans infection, supporting a possible role for StMKS2-3 in the response of potato S. tuberosum to such stress.
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Authors and Affiliations

Huong Thi Diem Tran
1
Thuong Thi Hong Nguye
1

  1. Faculty of Biology and Biotechnology, University of Science, Vietnam National University,Ho Chi Minh City, Nguyen Van Cu, Viet Nam
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Abstract

Agricultural production has a direct impact on the environment, both by consuming natural resources and by generating hazards in the form of emissions of various substances into the environment. Increased demand for plant products on global food markets contributes to heightened environmental pressure on agriculture. Agriculture, along with other sectors, should adhere to sustainability principles. Ongoing global development hinges on achieving a balance between economic growth and natural resource conservation. To fulfill the goals of sustainable development, agriculture should strive to minimize energy and natural resources consumption, thereby reducing its environmental impact. In the above context, research on the environmental effects of different agricultural production systems is needed. The aim of this study was to assess the environmental effects of two cultivation systems, conventional and organic, throughout the life cycles of winter wheat and potato production. The research employed a life cycle assessment (LCA) methodology from cradle to farm gate for assessing environmental impacts of crop cultivation across different farming systems, with respect to the functional unit of 1 tonne. Organic farming was shown to have lower environmental impacts than a conventional production system. The results confirm the sustainable nature of organic farming and its ability to mitigate the effects of farming activities. The LCA of conventional wheat and potato production showed that fertilizer application was the main environmental concern, highlighting the need to optimize fertilization to reduce environmental impacts. Furthermore, the results indicated that acidification and depletion of abiotic fossil fuel resources were significant environmental threats within the systems analyzed.
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Authors and Affiliations

Małgorzata Holka
1
Jolanta Kowalska
1
ORCID: ORCID

  1. Department of Organic Agriculture and Environmental Protection, Institute of Plant Protection − National Research Institute,Poznań, Poland

Instructions for authors

Instructions for Authors

Manuscripts published in JPPR are free of charge. Only colour figures and photos are payed 61.5 € per one colour page JPPR publishes original research papers, short communications, critical reviews, and book reviews covering all areas of modern plant protection. Subjects include phytopathological virology, bacteriology, mycology and applied nematology and entomology as well as topics on protecting crop plants and stocks of crop products against diseases, viruses, weeds, etc. Submitted manuscripts should provide new facts or confirmatory data. All manuscripts should be written in high-quality English. Non-English native authors should seek appropriate help from English-writing professionals before submission. The manuscript should be submitted only via the JPPR Editorial System (http://www.editorialsystem.com/jppr). The authors must also remember to upload a scan of a completed License to Publish (point 4 and a handwritten signature are of particular importance). ALP form is available at the Editorial System. The day the manuscript reaches the editors for the first time is given upon publication as the date ‘received’ and the day the version, corrected by the authors is accepted by the reviewers, is given as the date ‘revised’. All papers are available free of charge at the Journal’s webpage (www.plantprotection.pl). However, colour figures and photos cost 61.5 € per one colour page.

General information for preparing a manuscript

All text should be written in a concise and integrated way, by focusing on major points, findings, breakthrough or discoveries, and their broad significance. All running text should be in Times New Roman 12, 1.5 spacing with all margins 2.5 cm on all sides.

Original article

The original research articles should contain the following sections: Title – the title should be unambiguous, understandable to specialists in other fields, and must reflect the contents of the paper. No abbreviations may be used in the title. Name(s) of author(s) with affiliations footnoted added only to the system, not visible in the manuscript (Double Blind Reviews). The names of the authors should be given in the following order: first name, second name initial, surname. Affiliations should contain: name of institution, faculty, department, street, city with zip code, and country. Abstract – information given in the title does not need to be repeated in the abstract. The abstract should be no longer than 300 words. It must contain the aim of the study, methods, results and conclusions. If used, abbreviations should be limited and must be explained when first used. Keywords – a maximum of 6, should cover the most specific terms found in the paper. They should describe the subject and results and must differ from words used in the title. Introduction – a brief review of relevant research (with references to the most important and recent publications) should lead to the clear formulation of the working hypothesis and aim of the study. It is recommended to indicate what is novel and important in the study. Materials and Methods – in this section the description of experimental procedures should be sufficient to allow replication. Organisms must be identified by scientific name, including authors. The International System of Units (SI) and their abbreviations should be used. Methods of statistical processing, including the software used, should also be listed in this section. Results – should be presented clearly and concisely without deducting and theori sing. Graphs should be preferred over tables to express quantitative data. Discussion – should contain an interpretation of the results ( without unnecessary repetition) and explain the influence of experimental factors or methods. It should describe how the results and their interpretation relate to the scientific hypothesis and/or aim of the study. The discussion should take into account the current state of knowledge and up-to-date literature. It should highlight the significance and novelty of the paper. It may also point to the next steps that will lead to a better understanding of the matters in question. Acknowledgements – of people, grants, funds, etc. should be placed in a separate section before the reference list. The names of funding organizations should be written in full. References In the text, papers with more than two authors should be cited by the last name of the first author, followed by et al. (et al. in italics), a space, and the year of publication (example: Smith et al. 2012). If the cited manuscript has two authors, the citation should include both last names, a space, and the publication year (example: Marconi and Johnston 2006). In the Reference section, a maximum of ten authors of the cited paper may be given. All references cited in the text must be listed in the Reference section alphabetically by the last names of the author(s) and then chronologically. The year of publication follows the authors’ names. All titles of the cited articles should be given in English. Please limit the citation of papers published in languages other than English. If necessary translate the title into English and provide information concerning the original language in brackets (e.g. in Spanish). The list of references should only include works from the last ten years that have had the greatest impact on the subject. Older references can be cited only if they are important for manuscript content. The full name of periodicals should be given. If possible, the DOI number should be added at the end of each reference. The following system for arranging references should be used: Journal articles Jorjani M., Heydari A., Zamanizadeh H.R., Rezaee S., Naraghi L., Zamzami P. 2012. Controlling sugar beet mortality disease by application of new bioformulations. Journal of Plant Protection Research 52 (3): 303-307. DOI: https://doi.org/10.2478/v10045-012-0049-9 Online articles Turner E., Jacobson D.J., Taylor J.W. 2011. Genetic architecture of a reinforced, postmating, reproductive isolation barrier between Neurospora species indicates evolution via natural selection. PLoS Genetics 7 (8): e1002204. DOI: https://doi.org/10.1371/journal.pgen.1002204 Books Bancrof J.D., Stevens A. 1996. Theory and Practice of Histological Techniques. 4th ed. Churchill Livingstone, Edinburgh, UK, 776 pp. Book chapters Pradhan S.K. 2000. Integrated pest management. p. 463-469. In: "IPM System in Agriculture. Cash Crop" (R.K. Upadhyaya, K.G. Mukerji, O.P. Dubey, eds.). Aditya Books Pvt. Ltd. New Delhi, India, 710 pp. Online documents Cartwright J. 2007. Big stars have weather too. IOP Publishing PhysicsWeb. Available on: https://doi.org/10.1371/journal.pgen.1002204

Tables, Figures, Phothographs, Drawings

Tables and figures should be uploaded as separated files at the submission stage. Their place in the manuscript should be clearly indicated by authors. Colour figures are accepted at no charge for the electronic version. In the hardcopy version of the journal, colour figures cost (65,5 € per one colour page). When attaching files please indicate if you want colour only in the online version or in both the online and the hardcopy. Photographs and RGB bitmaps should be provided in JPG or TIFF file format. They must have no less than 300 dpi resolution. The text column should be 8 cm wide and they must be at least 1000 pixels wide. Please send original (not resized) photograph(s), straight from a digital camera, without any text descriptions on the photo. Bitmaps combined with text object descriptions should be provided in MS Word or MS Powerpoint format. Text objects using Arial font-face should be editable (changing font-face or font size). Drawings should be provided in MS Word, MS Powerpoint, CorelDRAW or EPS file format and stored with original data file. Text objects using Arial font-face should be editable (changing font-face or font size). Charts (MS Excel graphs) should be provided in MS Excel file format, and stored with original MS Excel data file without captions but with the number of the figure attached. Please do not use bitmap fills for bar charts. Use colour fills only if necessary. Captions and legends should be added at the end of the text, referred to as "Fig." and numbered consecutively throughout the paper.

Rapid communications

Rapid communications should present brief observations which do not warrant the length of a full paper. However, they must present completed studies and follow the same scientific standards as original articles. Rapid communications should contain the following sections: Title Abstract - less than 300 words Key words - maximum 6 Text body Acknowledgements References The length of such submissions is limited to 1500 words for the text, one table, and one figure.

Reviews

Review articles are invited by the editors.Unsolicited reviews are also considered. The length is limited to 5000 words with no limitations on figures and tables and a maximum of 150 references. Mini-Review articles should be dedicated to "hot" topics and limited to 3000 words and a maximum two figures, two tables and 20 references.

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